Anti-Estrogen Receptor beta抗体(ab5786)


  • 产品名称Anti-Estrogen Receptor beta抗体
    参阅全部 Estrogen Receptor beta 一抗
  • 描述
    兔多克隆抗体to Estrogen Receptor beta
  • 经测试应用适用于: WB, ICC, IHC-Fr, IHC-Pmore details
  • 种属反应性
    与反应: Rat, Human, Pig, Lizard, Non human primates, Baboon
  • 免疫原

    Synthetic peptide corresponding to Human Estrogen Receptor beta aa 459-477.


    (Peptide available as ab41764)

  • 阳性对照
    • WB: rat brain lysate IHC: rat prostate



Our Abpromise guarantee covers the use of ab5786 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 59 kDa).Can be blocked with Human Estrogen Receptor beta peptide (ab41764).
ICC Use at an assay dependent concentration.
IHC-Fr Use a concentration of 2 - 5 µg/ml.
IHC-P Use a concentration of 2 - 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.


  • 功能Nuclear hormone receptor. Binds estrogens with an affinity similar to that of ESR1, and activates expression of reporter genes containing estrogen response elements (ERE) in an estrogen-dependent manner. Isoform beta-cx lacks ligand binding ability and has no or only very low ere binding activity resulting in the loss of ligand-dependent transactivation ability. DNA-binding by ESR1 and ESR2 is rapidly lost at 37 degrees Celsius in the absence of ligand while in the presence of 17 beta-estradiol and 4-hydroxy-tamoxifen loss in DNA-binding at elevated temperature is more gradual.
  • 组织特异性Isoform beta-1 is expressed in testis and ovary, and at a lower level in heart, brain, placenta, liver, skeletal muscle, spleen, thymus, prostate, colon, bone marrow, mammary gland and uterus. Also found in uterine bone, breast, and ovarian tumor cell lines, but not in colon and liver tumors. Isoform beta-2 is expressed in spleen, thymus, testis and ovary and at a lower level in skeletal muscle, prostate, colon, small intestine, leukocytes, bone marrow, mammary gland and uterus. Isoform beta-3 is found in testis. Isoform beta-4 is expressed in testis, and at a lower level in spleen, thymus, ovary, mammary gland and uterus. Isoform beta-5 is expressed in testis, placenta, skeletal muscle, spleen and leukocytes, and at a lower level in heart, lung, liver, kidney, pancreas, thymus, prostate, colon, small intestine, bone marrow, mammary gland and uterus. Not expressed in brain.
  • 序列相似性Belongs to the nuclear hormone receptor family. NR3 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • 结构域Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ER BETA antibody
    • ER-beta antibody
    • Erb antibody
    • ESR B antibody
    • ESR BETA antibody
    • ESR2 antibody
    • ESR2_HUMAN antibody
    • ESRB antibody
    • ESTRB antibody
    • estrogen nuclear receptor beta variant a antibody
    • estrogen nuclear receptor beta variant b antibody
    • estrogen receptor 2 (ER beta) antibody
    • Estrogen receptor 2 antibody
    • estrogen receptor beta 4 antibody
    • Estrogen receptor beta antibody
    • NR3A2 antibody
    • Nuclear receptor subfamily 3 group A member 2 antibody
    see all

Anti-Estrogen Receptor beta antibody 图像

  • Immunohistochemical staining with ab5786 detects nuclear Estrogen Receptor beta in rat prostate

Anti-Estrogen Receptor beta antibody (ab5786)参考文献

This product has been referenced in:
  • Lloyd MC  et al. Vascular measurements correlate with estrogen receptor status. BMC Cancer 14:279 (2014). IHC-P ; Human . Read more (PubMed: 24755315) »
  • Anagnostis A & Papadopoulos AI Effects of a diet rich in sesame ( Sesamum indicum) pericarp on the expression of oestrogen receptor alpha and oestrogen receptor beta in rat prostate and uterus. Br J Nutr 102:703-8 (2009). WB ; Rat . Read more (PubMed: 19309532) »

See all 2 Publications for this product

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Thank you for your inquiry. My colleague will send you a proforma invoice shortly.

In regards to your question of staining: One vial of ab5786 contains 50ul. The optimal dilution should be determined in your laboratory. Lets suppose you will ...

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Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Human breast cancer xenograft(MCF-7))
Specification Human breast cancer xenograft(MCF-7)
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate buffer, pH7.0
Permeabilization No
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C

Abcam user community

Verified customer

提交于 Sep 17 2010

Thank you for your enquiry. I'm sorry ab5786 is giving your customer difficulty. From the protocol you give me I think the antigen retrieval method is fine and the incubation time/temperature for the primary antibody is fine too. I would check w...

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