Anti-Estrogen Receptor beta抗体(ab5784)

概述

  • 产品名称Anti-Estrogen Receptor beta抗体
    参阅全部 Estrogen Receptor beta 一抗
  • 描述
    兔多克隆抗体to Estrogen Receptor beta
  • 经测试应用适用于: WB, ICC/IF, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to Mouse Estrogen Receptor beta aa 467-485.
    Sequence:

    CCSTEDSKNKEGSQNLQSQ

  • 阳性对照
    • WB: rat brain lysate IHC: rat cerebellum (paraventricular nucleus and retrochiasmatic nucleus)

性能

应用

Our Abpromise guarantee covers the use of ab5784 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 2 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 59 kDa).
ICC/IF 1/50 - 1/200.
IHC-P Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.

靶标

  • 功能Nuclear hormone receptor. Binds estrogens with an affinity similar to that of ESR1, and activates expression of reporter genes containing estrogen response elements (ERE) in an estrogen-dependent manner. Isoform beta-cx lacks ligand binding ability and has no or only very low ere binding activity resulting in the loss of ligand-dependent transactivation ability. DNA-binding by ESR1 and ESR2 is rapidly lost at 37 degrees Celsius in the absence of ligand while in the presence of 17 beta-estradiol and 4-hydroxy-tamoxifen loss in DNA-binding at elevated temperature is more gradual.
  • 组织特异性Isoform beta-1 is expressed in testis and ovary, and at a lower level in heart, brain, placenta, liver, skeletal muscle, spleen, thymus, prostate, colon, bone marrow, mammary gland and uterus. Also found in uterine bone, breast, and ovarian tumor cell lines, but not in colon and liver tumors. Isoform beta-2 is expressed in spleen, thymus, testis and ovary and at a lower level in skeletal muscle, prostate, colon, small intestine, leukocytes, bone marrow, mammary gland and uterus. Isoform beta-3 is found in testis. Isoform beta-4 is expressed in testis, and at a lower level in spleen, thymus, ovary, mammary gland and uterus. Isoform beta-5 is expressed in testis, placenta, skeletal muscle, spleen and leukocytes, and at a lower level in heart, lung, liver, kidney, pancreas, thymus, prostate, colon, small intestine, bone marrow, mammary gland and uterus. Not expressed in brain.
  • 序列相似性Belongs to the nuclear hormone receptor family. NR3 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • 结构域Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ER BETA antibody
    • ER-beta antibody
    • Erb antibody
    • ESR B antibody
    • ESR BETA antibody
    • ESR2 antibody
    • ESR2_HUMAN antibody
    • ESRB antibody
    • ESTRB antibody
    • estrogen nuclear receptor beta variant a antibody
    • estrogen nuclear receptor beta variant b antibody
    • estrogen receptor 2 (ER beta) antibody
    • Estrogen receptor 2 antibody
    • estrogen receptor beta 4 antibody
    • Estrogen receptor beta antibody
    • NR3A2 antibody
    • Nuclear receptor subfamily 3 group A member 2 antibody
    see all

Anti-Estrogen Receptor beta antibody 图像

  • Immunocytochemistry/Immunofluorescence analysis of HeLa cells labeling Estrogen Receptor beta (green) with ab5784 at 1/100. Cells were fixed with formalin, permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 5% normal goat serum for 15 minutes at room temperature. Cells were incubated with the primary antibody for at least 1 hour at room temperature. A DyLight 488-conjugated goat anti-rabbit IgG (1/200) secondary antibody was used. Nuclei (blue) were stained with Hoechst. 20X magnification.



  • Predicted band size : 59 kDa

    Shows a Western blot of Estrogen Receptor beta on HEK cell extract using ab5784.

Anti-Estrogen Receptor beta antibody (ab5784)参考文献

ab5784 has not yet been referenced specifically in any publications.

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