Anti-Estrogen Receptor beta抗体(ab3576)


  • 产品名称Anti-Estrogen Receptor beta抗体
    参阅全部 Estrogen Receptor beta 一抗
  • 描述
    兔多克隆抗体to Estrogen Receptor beta
  • 特异性By Western blot, this antibody detects an ~55 kDa protein representing ER beta from Rat brain homogenate. Immunocytochemical staining of ER beta in cells overexpressing the Human protein results in nuclear staining.
  • 经测试应用适用于: IHC-P, IHC-Fr, ICC, WB, Inhibition Assay, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Sheep, Human, Pig, Non Human Primates
  • 免疫原

    Synthetic peptide corresponding to Rat Estrogen Receptor beta aa 467-485.


    (Peptide available as ab5018)

  • 阳性对照
    • WB: Rat brain lysate IHC: testis, efferent ductules, epididymis, and vas deferens (see Zhou et al), Rat ovary (see Sanchez-Criado)



Our Abpromise guarantee covers the use of ab3576 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/50. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.
IHC-Fr 1/50.
ICC 1/100 - 1/1000.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 59 kDa).Can be blocked with Estrogen Receptor beta peptide (ab5018).
Inhibition Assay Use at an assay dependent concentration.
ICC/IF 1/100 - 1/1000.


  • 功能Nuclear hormone receptor. Binds estrogens with an affinity similar to that of ESR1, and activates expression of reporter genes containing estrogen response elements (ERE) in an estrogen-dependent manner. Isoform beta-cx lacks ligand binding ability and has no or only very low ere binding activity resulting in the loss of ligand-dependent transactivation ability. DNA-binding by ESR1 and ESR2 is rapidly lost at 37 degrees Celsius in the absence of ligand while in the presence of 17 beta-estradiol and 4-hydroxy-tamoxifen loss in DNA-binding at elevated temperature is more gradual.
  • 组织特异性Isoform beta-1 is expressed in testis and ovary, and at a lower level in heart, brain, placenta, liver, skeletal muscle, spleen, thymus, prostate, colon, bone marrow, mammary gland and uterus. Also found in uterine bone, breast, and ovarian tumor cell lines, but not in colon and liver tumors. Isoform beta-2 is expressed in spleen, thymus, testis and ovary and at a lower level in skeletal muscle, prostate, colon, small intestine, leukocytes, bone marrow, mammary gland and uterus. Isoform beta-3 is found in testis. Isoform beta-4 is expressed in testis, and at a lower level in spleen, thymus, ovary, mammary gland and uterus. Isoform beta-5 is expressed in testis, placenta, skeletal muscle, spleen and leukocytes, and at a lower level in heart, lung, liver, kidney, pancreas, thymus, prostate, colon, small intestine, bone marrow, mammary gland and uterus. Not expressed in brain.
  • 序列相似性Belongs to the nuclear hormone receptor family. NR3 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • 结构域Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ER BETA antibody
    • ER-beta antibody
    • Erb antibody
    • ESR B antibody
    • ESR BETA antibody
    • ESR2 antibody
    • ESR2_HUMAN antibody
    • ESRB antibody
    • ESTRB antibody
    • estrogen nuclear receptor beta variant a antibody
    • estrogen nuclear receptor beta variant b antibody
    • estrogen receptor 2 (ER beta) antibody
    • Estrogen receptor 2 antibody
    • estrogen receptor beta 4 antibody
    • Estrogen receptor beta antibody
    • NR3A2 antibody
    • Nuclear receptor subfamily 3 group A member 2 antibody
    see all

Anti-Estrogen Receptor beta antibody 图像

  • Immunofluorescent analysis using Estrogen Receptor beta polyclonal antibody (ab3576) shows nuclear localization of Estrogen Receptor beta in MCF7 cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature. Cells were blocked with 1% BSA for 15 minutes at room temperature. Cells were probed with a rabbit polyclonal antibody recognizing Estrogen Receptor beta (ab3576), at a dilution of 1:100 for 1 hour at room temperature. Cells were washed with PBS and incubated with DyLight 488 goat-anti-rabbit IgG secondary antibody at a dilution of 1:400 for 30 minutes at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye. Images were taken at 20X magnification.
  • All lanes : Anti-Estrogen Receptor beta antibody (ab3576) at 1/2000 dilution

    Lane 1 : CAL region of Human brain - Whole cell lysate
    Lane 2 : EC region of Human brain - Whole cell lysate
    Lane 3 : HIP region of Human brain - Whole cell lysate
    Lane 4 : IT region of Human brain - Whole cell lysate
    Lane 5 : MF region of Human brain - Whole cell lysate
    Lane 6 : SP region of Human brain - Whole cell lysate

    HRP conjugated Donkey Anti-rabbit IgG

    Predicted band size : 59 kDa
    Observed band size : 55 kDa (why is the actual band size different from the predicted?)
    This image is courtesy of an Abreview submitted on 3 February 2006.

    See Abreview

  • Immunolocalization of ER beta from CHO cells overexpressing the Human protein, using ab3576 (1ug/ml).

  • Immunofluorescence analysis of frozen murine thoracic aorta, staining Estrogen Receptor beta with ab3576 at 1/50 dilution (green: not merged with nucleus, or purple: merged with nucleus). Nuclei were stained with DAPI (blue) and actin fibers were stained red.

Anti-Estrogen Receptor beta antibody (ab3576)参考文献

This product has been referenced in:
  • Singh V  et al. Apigenin manipulates the ubiquitin-proteasome system to rescue estrogen receptor-ß from degradation and induce apoptosis in prostate cancer cells. Eur J Nutr N/A:N/A (2014). Read more (PubMed: 25408199) »
  • Ishii T  et al. Genetically induced oxidative stress in mice causes thrombocytosis, splenomegaly and placental angiodysplasia that leads to recurrent abortion. Redox Biol 2:679-85 (2014). WB ; Mouse . Read more (PubMed: 24936442) »

See all 9 Publications for this product

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I can confirm that ab3576: Anti-Estrogen Receptor beta has been used in different dilutions in IHC (P).

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Application Flow Cytometry
Sample Rat Cell (Lung tissue)
Permeabilization Yes - eBiosciences FoxP3 intracellular staining buffer set
Gating Strategy Leukocytes
Specification Lung tissue
Preparation Cell harvesting/tissue preparation method: Lungs were dissected and digested using Collagenase IV+DNase
Sample buffer: GKN+10% FCS
Fixation eBiosciences FoxP3 intracellular staining buffer set

Abcam user community

Verified customer

提交于 Jan 12 2016

Thank you for taking the time to contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.
The details you have kindly provided will enable us to investigate this case for you and this is also helpfu...

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Thank you very much for you reply.

I'm sending a free of charge vial of ab3576 on the order ***, which we expect to arrive around August 24. Your purchasing agent will receive an email update if there are further delays to expect.


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Thank you again for your call yesterday and for letting us know about the trouble with ab3577.

I've checked with our supply chain and we do expect to have ab3576 back in stock by mid-late August, tentatively August 21. Would this work for you...

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Thank you very much for your call today and for letting us know about the trouble with ab3577.

As we discussed, I will be happy to send a free of charge replacement antibody since this one did not work. I'm checking with our supply chain rega...

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Thank you for your reply. I am sorry that ab3576 was also producing high background. I have arranged to send you ab3577 as a replacement and am also sending a positive control mouse brain nuclear lysate, which may help to clarify your results. The repl...

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I am sorry that ab16813 is still producing multiple bands in WB. I would be happy to send you ab3576 or ab3577 as a free of charge replacement, as we had previously discussed. Please let me know your original order o...

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