Anti-Estrogen Receptor beta抗体[14C8] (ab288)

概述

  • 产品名称Anti-Estrogen Receptor beta抗体[14C8]
    参阅全部 Estrogen Receptor beta 一抗
  • 描述
    小鼠单克隆抗体[14C8] to Estrogen Receptor beta
  • 特异性14C8 is specific for human ER-beta. The antibody has been tested in WB against recombinant protein. It has not yet been tested in our laboratory using any cell lysates and several publications suggest it can give weak bands in breast tumour extracts lysate. Strong Erb staining in epithelial cell nuclei. Occasional weak to moderate staining is seen in surrounding stromal and endothelial cell nuclei. Sporadic light cytoplasmic staining is sometimes observed. We have also successfully detected ERb in colon and ovarian tumors using the same antibody.
  • 经测试应用适用于: WB, Flow Cyt, IHC-P, ICC/IF, ChIP, IHC-Frmore details
  • 种属反应性
    与反应: Mouse, Human, Baboon
  • 免疫原

    Recombinant 6-His fusion protein encoding amino acids 1-153 of human ER-beta expressed in E. coli.

  • 阳性对照
    • WB: Recombinant fusion protein. IHC-P: endocervical epithelium, Lobular breast carcinoma.

性能

应用

Our Abpromise guarantee covers the use of ab288 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 59 kDa.
Flow Cyt 1/25.
IHC-P Use a concentration of 5 µg/ml.
ICC/IF Use at an assay dependent concentration.
ChIP Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.

靶标

  • 功能Nuclear hormone receptor. Binds estrogens with an affinity similar to that of ESR1, and activates expression of reporter genes containing estrogen response elements (ERE) in an estrogen-dependent manner. Isoform beta-cx lacks ligand binding ability and has no or only very low ere binding activity resulting in the loss of ligand-dependent transactivation ability. DNA-binding by ESR1 and ESR2 is rapidly lost at 37 degrees Celsius in the absence of ligand while in the presence of 17 beta-estradiol and 4-hydroxy-tamoxifen loss in DNA-binding at elevated temperature is more gradual.
  • 组织特异性Isoform beta-1 is expressed in testis and ovary, and at a lower level in heart, brain, placenta, liver, skeletal muscle, spleen, thymus, prostate, colon, bone marrow, mammary gland and uterus. Also found in uterine bone, breast, and ovarian tumor cell lines, but not in colon and liver tumors. Isoform beta-2 is expressed in spleen, thymus, testis and ovary and at a lower level in skeletal muscle, prostate, colon, small intestine, leukocytes, bone marrow, mammary gland and uterus. Isoform beta-3 is found in testis. Isoform beta-4 is expressed in testis, and at a lower level in spleen, thymus, ovary, mammary gland and uterus. Isoform beta-5 is expressed in testis, placenta, skeletal muscle, spleen and leukocytes, and at a lower level in heart, lung, liver, kidney, pancreas, thymus, prostate, colon, small intestine, bone marrow, mammary gland and uterus. Not expressed in brain.
  • 序列相似性Belongs to the nuclear hormone receptor family. NR3 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • 结构域Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ER BETA antibody
    • ER-beta antibody
    • Erb antibody
    • ESR B antibody
    • ESR BETA antibody
    • ESR2 antibody
    • ESR2_HUMAN antibody
    • ESRB antibody
    • ESTRB antibody
    • estrogen nuclear receptor beta variant a antibody
    • estrogen nuclear receptor beta variant b antibody
    • estrogen receptor 2 (ER beta) antibody
    • Estrogen receptor 2 antibody
    • estrogen receptor beta 4 antibody
    • Estrogen receptor beta antibody
    • NR3A2 antibody
    • Nuclear receptor subfamily 3 group A member 2 antibody
    see all

Anti-Estrogen Receptor beta antibody [14C8] 图像

  • ab288 staining endocervical epithelium by IHC-P.
  • ab288 staining infiltrating lobular carcinoma of the breast by IHC-P.

Anti-Estrogen Receptor beta antibody [14C8] (ab288)参考文献

This product has been referenced in:
  • Tan WJ  et al. MED12 protein expression in breast fibroepithelial lesions: correlation with mutation status and oestrogen receptor expression. J Clin Pathol 69:858-65 (2016). Read more (PubMed: 27056456) »
  • Song W  et al. Infiltrating neutrophils promote renal cell carcinoma progression via VEGFa/HIF2a and estrogen receptor ß signals. Oncotarget 6:19290-304 (2015). IHC-P . Read more (PubMed: 26079540) »

See all 39 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (OV90)
Permeabilization Yes - 0.25% Triton-X100 in PBS
Specification OV90
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C
Fixative Formaldehyde
Username

Abcam user community

Verified customer

提交于 Oct 26 2016

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 10mM sodium citrate, pH 6.0
Sample Baboon Tissue sections (fetal ovary)
Specification fetal ovary
Permeabilization No
Fixative Formaldehyde
Username

Ms. Marcia Burch

Verified customer

提交于 Sep 25 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Breast cancer)
Specification Breast cancer
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: TRIS-EDTA-Buffer pH 9,0
Permeabilization Yes - Wash Buffer with tween
Username

Mr. Rudolf Jung

Verified customer

提交于 Dec 03 2012

Thank you for your enquiry.

I can confirm that we recommend not to dilute antibodies before storage unless is states to do so on the individual datasheet. For all antibodies, we suggest to always follow instructions provided on the individua...

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Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.


Please apologize for the delay of my answer: Anja is on her well deserved holiday now, so I ...

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Thank you for your enquiry and your interest in our products.
The effect of formalin fixation and the long-term storage on the detectability of different proteins have been studies for some time. The longer the tissues are fixed and stored in form...

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Thank you for taking time to liaise with this customer to complete our questionnaire. I am sorry to hear that this antibody is not providing satisfactory results. Having reviewed this case, I would like to offer some suggestions to help optimize the re...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Human Tissue sections (vaginal)
Specification vaginal
Fixative Paraformaldehyde
Permeabilization Yes - 0.2% Triton in PBS
Blocking step (agent) for 30 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Oct 17 2011

Thank you for your inquiry. The 6x his tag was not removed before the antibody was raised. If I can help provide any further information please let me know.

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (breast cancer)
Specification breast cancer
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: • Microwave slides in 10mM citrate buffer pH 6.0
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Username

Dr. W Xu

Verified customer

提交于 May 11 2010

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