Anti-Estrogen Receptor alpha (phospho Y537)抗体(ab59177)

概述

  • 产品名称Anti-Estrogen Receptor alpha (phospho Y537)抗体
    参阅全部 Estrogen Receptor alpha 一抗
  • 描述
    兔多克隆抗体to Estrogen Receptor alpha (phospho Y537)
  • 特异性Detects endogenous levels of Estrogen Receptor alpha only when phosphorylated at tyrosine 537.
  • 经测试应用适用于: WB, IHC-P, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse
  • 免疫原

    Synthetic phosphopeptide from around the phosphorylation site of tyrosine 537 (PLYPDL) of human Estrogen Receptor alpha

  • 阳性对照
    • Human lung carcinoma tissue. 293 cell extracts.

性能

应用

Our Abpromise guarantee covers the use of ab59177 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa).
IHC-P Use at an assay dependent concentration.
ELISA 1/5000.

靶标

  • 功能Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Can activate the transcriptional activity of TFF1.
  • 序列相似性Belongs to the nuclear hormone receptor family. NR3 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • 结构域Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
  • 翻译后修饰Phosphorylated by cyclin A/CDK2. Phosphorylation probably enhances transcriptional activity.
    Glycosylated; contains N-acetylglucosamine, probably O-linked.
    Ubiquitinated. Deubiquitinated by OTUB1.
    Dimethylated by PRMT1 at Arg-260. The methylation may favor cytoplasmic localization.
    Palmitoylated (isoform 3). Not biotinylated (isoform 3).
  • 细胞定位Nucleus. Cytoplasm. Cell membrane. A minor fraction is associated with the inner membrane and Nucleus. Cytoplasm. Cell membrane. Associated with the inner membrane via palmitoylation.
  • Information by UniProt
  • 数据库链接
  • 别名
    • DKFZp686N23123 antibody
    • ER alpha antibody
    • ER antibody
    • ER-alpha antibody
    • Era antibody
    • ESR antibody
    • ESR1 antibody
    • ESR1_HUMAN antibody
    • ESRA antibody
    • Estradiol receptor antibody
    • Estrogen nuclear receptor alpha antibody
    • Estrogen receptor 1 antibody
    • Estrogen receptor alpha 3*,4,5,6,7*/822 isoform antibody
    • Estrogen receptor alpha antibody
    • Estrogen receptor alpha delta 3*,4,5,6,7*,8*/941 isoform antibody
    • Estrogen receptor alpha delta 3*,4,5,6,7*/819 2 isoform antibody
    • Estrogen receptor alpha delta 4 +49 isoform antibody
    • Estrogen receptor alpha delta 4*,5,6,7*/654 isoform antibody
    • Estrogen receptor alpha delta 4*,5,6,7,8*/901 isoform antibody
    • Estrogen receptor alpha E1 E2 1 2 antibody
    • Estrogen receptor alpha E1 N2 E2 1 2 antibody
    • Estrogen receptor antibody
    • ESTRR antibody
    • NR3A1 antibody
    • Nuclear receptor subfamily 3 group A member 1 antibody
    see all

Anti-Estrogen Receptor alpha (phospho Y537) antibody 图像

  • Immunohistochemical analysis of paraffin embedded human lung carcinoma tissue using ab59177 at 1/50 dilution. Left: untreated. Right: sample treated with immunising peptide.
  • All lanes : Anti-Estrogen Receptor alpha (phospho Y537) antibody (ab59177) at 1/500 dilution

    Lane 1 : 293 cell extract
    Lane 2 : 293 cell extract with phosphopeptide


    Predicted band size : 68 kDa
    Observed band size : 68 kDa

Anti-Estrogen Receptor alpha (phospho Y537) antibody (ab59177)参考文献

ab59177 has not yet been referenced specifically in any publications.

Product Wall

Application ChIP
Detection step Real-time PCR
Sample Human Cell lysate - nuclear (Breast Cancer)
Specification Breast Cancer
Type Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: Formaldehyde
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提交于 Mar 25 2015

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