Anti-Estrogen Receptor alpha (phospho S118)抗体(ab31477)

概述

  • 产品名称
    Anti-Estrogen Receptor alpha (phospho S118)抗体
    参阅全部 Estrogen Receptor alpha 一抗
  • 描述
    兔多克隆抗体to Estrogen Receptor alpha (phospho S118)
  • 特异性
    ab31477 detects endogenous levels of Estrogen Receptor-alpha only when phosphorylated at Serine 118.
  • 经测试应用
    适用于: WB, IHC-P, ELISAmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide (Human) - derived from around phosphorylation site at Serine118.

  • 阳性对照
    • MCF7 cell extracts, breast carcinoma.

性能

应用

Our Abpromise guarantee covers the use of ab31477 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Predicted molecular weight: 68 kDa.
IHC-P 1/50 - 1/100.
ELISA 1/20000.

靶标

  • 功能
    Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Can activate the transcriptional activity of TFF1.
  • 序列相似性
    Belongs to the nuclear hormone receptor family. NR3 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • 结构域
    Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
  • 翻译后修饰
    Phosphorylated by cyclin A/CDK2. Phosphorylation probably enhances transcriptional activity.
    Glycosylated; contains N-acetylglucosamine, probably O-linked.
    Ubiquitinated. Deubiquitinated by OTUB1.
    Dimethylated by PRMT1 at Arg-260. The methylation may favor cytoplasmic localization.
    Palmitoylated (isoform 3). Not biotinylated (isoform 3).
  • 细胞定位
    Nucleus. Cytoplasm. Cell membrane. A minor fraction is associated with the inner membrane and Nucleus. Cytoplasm. Cell membrane. Associated with the inner membrane via palmitoylation.
  • Information by UniProt
  • 数据库链接
  • 别名
    • DKFZp686N23123 antibody
    • ER alpha antibody
    • ER antibody
    • ER-alpha antibody
    • Era antibody
    • ESR antibody
    • ESR1 antibody
    • ESR1_HUMAN antibody
    • ESRA antibody
    • Estradiol receptor antibody
    • Estrogen nuclear receptor alpha antibody
    • Estrogen receptor 1 antibody
    • Estrogen receptor alpha 3*,4,5,6,7*/822 isoform antibody
    • Estrogen receptor alpha antibody
    • Estrogen receptor alpha delta 3*,4,5,6,7*,8*/941 isoform antibody
    • Estrogen receptor alpha delta 3*,4,5,6,7*/819 2 isoform antibody
    • Estrogen receptor alpha delta 4 +49 isoform antibody
    • Estrogen receptor alpha delta 4*,5,6,7*/654 isoform antibody
    • Estrogen receptor alpha delta 4*,5,6,7,8*/901 isoform antibody
    • Estrogen receptor alpha E1 E2 1 2 antibody
    • Estrogen receptor alpha E1 N2 E2 1 2 antibody
    • Estrogen receptor antibody
    • ESTRR antibody
    • NR3A1 antibody
    • Nuclear receptor subfamily 3 group A member 1 antibody
    see all

Anti-Estrogen Receptor alpha (phospho S118) antibody 图像

  • Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Estrogen Receptor-alpha (Phospho-Ser118) Antibody (ab31477) . The picture on the right is treated with the synthesized peptide.
  • All lanes : Anti-Estrogen Receptor alpha (phospho S118) antibody (ab31477) at 1/1000 dilution

    Lane 1 : MCF7 cell extract -treated with E2
    Lane 2 : MCF7 cell extract -untreated


    Predicted band size : 68 kDa
    Observed band size : 65 kDa (why is the actual band size different from the predicted?)

Anti-Estrogen Receptor alpha (phospho S118) antibody (ab31477)参考文献

This product has been referenced in:
  • Monsivais D  et al. Uterine ALK3 is essential during the window of implantation. Proc Natl Acad Sci U S A 113:E387-95 (2016). IHC-P ; Mouse . Read more (PubMed: 26721398) »
  • Su RW  et al. Aberrant activation of canonical Notch1 signaling in the mouse uterus decreases progesterone receptor by hypermethylation and leads to infertility. Proc Natl Acad Sci U S A 113:2300-5 (2016). Read more (PubMed: 26858409) »

See all 3 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (MCF7 and 293)
Loading amount
1e+006 cells
Specification
MCF7 and 293
Treatment
293 not treated, MCF7 ss and stimulated with E2
Gel Running Conditions
Reduced Denaturing (4-15% gradient gel)
Blocking step
Odyssey blocking buffer as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 23°C
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提交于 Jul 26 2012

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