Anti-Estrogen Receptor alpha (phospho S104 + S106)抗体(ab51107)

概述

  • 产品名称Anti-Estrogen Receptor alpha (phospho S104 + S106)抗体
    参阅全部 Estrogen Receptor alpha 一抗
  • 描述
    兔多克隆抗体to Estrogen Receptor alpha (phospho S104 + S106)
  • 特异性ab51107 detects endogenous levels of Estrogen Receptor alpha only when phosphorylated at serine 104/106.
  • 经测试应用适用于: IHC-P, WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse
  • 免疫原

    A synthetic phospho-peptide derived from human Estrogen Receptor alpha around the phosphorylation site of serine 104/106 (V-SP-P-SP-P).

  • 阳性对照
    • IHC-P: Human breast carcinoma tissue; WB: Estradiol- treated MCF7 cell lysates.

性能

应用

Our Abpromise guarantee covers the use of ab51107 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P
WB
ELISA
  • 应用说明ELISA: 1/10000.
    IHC-P: 1/50 - 1/100.
    WB: 1/500 - 1/1000. Predicted molecular weight (non-phosphorilated protein): 65 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Can activate the transcriptional activity of TFF1.
    • 序列相似性Belongs to the nuclear hormone receptor family. NR3 subfamily.
      Contains 1 nuclear receptor DNA-binding domain.
    • 结构域Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
    • 翻译后修饰Phosphorylated by cyclin A/CDK2. Phosphorylation probably enhances transcriptional activity.
      Glycosylated; contains N-acetylglucosamine, probably O-linked.
      Ubiquitinated. Deubiquitinated by OTUB1.
      Dimethylated by PRMT1 at Arg-260. The methylation may favor cytoplasmic localization.
      Palmitoylated (isoform 3). Not biotinylated (isoform 3).
    • 细胞定位Nucleus. Cytoplasm. Cell membrane. A minor fraction is associated with the inner membrane and Nucleus. Cytoplasm. Cell membrane. Associated with the inner membrane via palmitoylation.
    • Information by UniProt
    • 数据库链接
    • 别名
      • DKFZp686N23123 antibody
      • ER alpha antibody
      • ER antibody
      • ER-alpha antibody
      • Era antibody
      • ESR antibody
      • ESR1 antibody
      • ESR1_HUMAN antibody
      • ESRA antibody
      • Estradiol receptor antibody
      • Estrogen nuclear receptor alpha antibody
      • Estrogen receptor 1 antibody
      • Estrogen receptor alpha 3*,4,5,6,7*/822 isoform antibody
      • Estrogen receptor alpha antibody
      • Estrogen receptor alpha delta 3*,4,5,6,7*,8*/941 isoform antibody
      • Estrogen receptor alpha delta 3*,4,5,6,7*/819 2 isoform antibody
      • Estrogen receptor alpha delta 4 +49 isoform antibody
      • Estrogen receptor alpha delta 4*,5,6,7*/654 isoform antibody
      • Estrogen receptor alpha delta 4*,5,6,7,8*/901 isoform antibody
      • Estrogen receptor alpha E1 E2 1 2 antibody
      • Estrogen receptor alpha E1 N2 E2 1 2 antibody
      • Estrogen receptor antibody
      • ESTRR antibody
      • NR3A1 antibody
      • Nuclear receptor subfamily 3 group A member 1 antibody
      see all

    Anti-Estrogen Receptor alpha (phospho S104 + S106) antibody 图像

    • All lanes : Anti-Estrogen Receptor alpha (phospho S104 + S106) antibody (ab51107)

      Lane 1 : Untreated MCF7 cell lysate.
      Lane 2 : Estradiol-treated MCF7 cell lysate.


      Predicted band size : 65 kDa
    • ab51107 (1/100) staining Estrogen Receptor alpha (phospho S104 + S106) in paraffin embedded human breast carcinoma tissue by Immunohistochemistry. Right panel represents a negative control experiment done in the presence of the immunizing (blocking) phospho-peptide.

    Anti-Estrogen Receptor alpha (phospho S104 + S106) antibody (ab51107)参考文献

    ab51107 has not yet been referenced specifically in any publications.

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