Anti-Estrogen Receptor alpha (phospho S104)抗体(ab30656)

概述

  • 产品名称Anti-Estrogen Receptor alpha (phospho S104)抗体
    参阅全部 Estrogen Receptor alpha 一抗
  • 描述
    兔多克隆抗体to Estrogen Receptor alpha (phospho S104)
  • 特异性This antibody detects endogenous levels of Estrogen Receptor alpha only when phosphorylated at Serine 104.
  • 经测试应用适用于: WB, ELISA, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse
  • 免疫原

    Synthetic phosphopeptide derived from human Estrogen Receptor alpha around the phosphorylation site of Serine 104.

  • 阳性对照
    • Extracts of 293 cells treated with forskolin.

性能

应用

Our Abpromise guarantee covers the use of ab30656 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 60 kDa (predicted molecular weight: 65 kDa).
ELISA 1/4000.
IHC-P Use at an assay dependent concentration.

靶标

  • 功能Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Can activate the transcriptional activity of TFF1.
  • 序列相似性Belongs to the nuclear hormone receptor family. NR3 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • 结构域Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
  • 翻译后修饰Phosphorylated by cyclin A/CDK2. Phosphorylation probably enhances transcriptional activity.
    Glycosylated; contains N-acetylglucosamine, probably O-linked.
    Ubiquitinated. Deubiquitinated by OTUB1.
    Dimethylated by PRMT1 at Arg-260. The methylation may favor cytoplasmic localization.
    Palmitoylated (isoform 3). Not biotinylated (isoform 3).
  • 细胞定位Nucleus. Cytoplasm. Cell membrane. A minor fraction is associated with the inner membrane and Nucleus. Cytoplasm. Cell membrane. Associated with the inner membrane via palmitoylation.
  • Information by UniProt
  • 数据库链接
  • 别名
    • DKFZp686N23123 antibody
    • ER alpha antibody
    • ER antibody
    • ER-alpha antibody
    • Era antibody
    • ESR antibody
    • ESR1 antibody
    • ESR1_HUMAN antibody
    • ESRA antibody
    • Estradiol receptor antibody
    • Estrogen nuclear receptor alpha antibody
    • Estrogen receptor 1 antibody
    • Estrogen receptor alpha 3*,4,5,6,7*/822 isoform antibody
    • Estrogen receptor alpha antibody
    • Estrogen receptor alpha delta 3*,4,5,6,7*,8*/941 isoform antibody
    • Estrogen receptor alpha delta 3*,4,5,6,7*/819 2 isoform antibody
    • Estrogen receptor alpha delta 4 +49 isoform antibody
    • Estrogen receptor alpha delta 4*,5,6,7*/654 isoform antibody
    • Estrogen receptor alpha delta 4*,5,6,7,8*/901 isoform antibody
    • Estrogen receptor alpha E1 E2 1 2 antibody
    • Estrogen receptor alpha E1 N2 E2 1 2 antibody
    • Estrogen receptor antibody
    • ESTRR antibody
    • NR3A1 antibody
    • Nuclear receptor subfamily 3 group A member 1 antibody
    see all

Anti-Estrogen Receptor alpha (phospho S104) antibody 图像

  • ab30656 staining human Estrogen Receptor alpha in breast carcinoma by Immunohistochemistry, Paraffin embedded tissue.

    Left image antibody un-treated.

    Right image antibody pre-incubated with synthetic phosphopeptide.

  • All lanes : Anti-Estrogen Receptor alpha (phospho S104) antibody (ab30656) at 1 µg/ml

    Lane 1 : 293 cells treated with forskolin. Antibody un-treated.
    Lane 2 : 293 cells treated with forskolin. Antibody pre-incubated with synthetic phosphopeptide.

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 65 kDa

Anti-Estrogen Receptor alpha (phospho S104) antibody (ab30656)参考文献

This product has been referenced in:
  • Kalthoff S  et al. Gender matters: estrogen receptor alpha (ERa) and histone deacetylase (HDAC) 1 and 2 control the gender-specific transcriptional regulation of human uridine diphosphate glucuronosyltransferases genes (UGT1A). J Hepatol 59:797-804 (2013). IHC-P ; Human . Read more (PubMed: 23714156) »

See 1 Publication for this product

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Thank you for your enquiry. I apologise for the delay in obtaining this information for you. I can confirm that the specificity of antibody (ab30656) has been tested by using a blocking peptide in IHC. Also, when used in ELISA, the reading increase...

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Thank you for your enquiry. The immunogen for this ER alpha antibody is not conserved in ER beta. I hope this information helps. Please do not hesitate to contact us if you need anything further.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"