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Synthetic peptide corresponding to Human Estrogen Receptor alpha. Synthetic peptide derived from C-terminal of human estrogen receptor alpha.
Our Abpromise guarantee covers the use of ab27595 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration.|
|IHC-P||Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
USE NEAT. Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 min.
Immunohistochemical staining of human breast carcinoma labelling Estrogen Receptor alpha [SP1] with ab93739 at 1:200 dilution.