Anti-Estrogen Receptor alpha抗体[SP1],prediluted (ab27595)


  • 产品名称
    Anti-Estrogen Receptor alpha抗体[SP1],prediluted
    参阅全部 Estrogen Receptor alpha 一抗
  • 描述
    兔单克隆抗体[SP1] to Estrogen Receptor alpha,prediluted
  • 宿主
  • 特异性
    ab27595 recognises estrogen receptor alpha (ER).
  • 经测试应用
    适用于: IHC-FoFr, WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Pig
  • 免疫原

    Synthetic peptide corresponding to Human Estrogen Receptor alpha. Synthetic peptide derived from C-terminal of human estrogen receptor alpha.

  • 阳性对照
    • Breast carcinomas



Our Abpromise guarantee covers the use of ab27595 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-FoFr Use at an assay dependent concentration. PubMed: 29403069
WB Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

USE NEAT. Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 min.


  • 功能
    Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Can activate the transcriptional activity of TFF1.
  • 序列相似性
    Belongs to the nuclear hormone receptor family. NR3 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • 结构域
    Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
  • 翻译后修饰
    Phosphorylated by cyclin A/CDK2. Phosphorylation probably enhances transcriptional activity.
    Glycosylated; contains N-acetylglucosamine, probably O-linked.
    Ubiquitinated. Deubiquitinated by OTUB1.
    Dimethylated by PRMT1 at Arg-260. The methylation may favor cytoplasmic localization.
    Palmitoylated (isoform 3). Not biotinylated (isoform 3).
  • 细胞定位
    Nucleus. Cytoplasm. Cell membrane. A minor fraction is associated with the inner membrane and Nucleus. Cytoplasm. Cell membrane. Associated with the inner membrane via palmitoylation.
  • Information by UniProt
  • 数据库链接
  • 别名
    • DKFZp686N23123 antibody
    • ER alpha antibody
    • ER antibody
    • ER-alpha antibody
    • Era antibody
    • ESR antibody
    • ESR1 antibody
    • ESR1_HUMAN antibody
    • ESRA antibody
    • Estradiol receptor antibody
    • Estrogen nuclear receptor alpha antibody
    • Estrogen receptor 1 antibody
    • Estrogen receptor alpha 3*,4,5,6,7*/822 isoform antibody
    • Estrogen receptor alpha antibody
    • Estrogen receptor alpha delta 3*,4,5,6,7*,8*/941 isoform antibody
    • Estrogen receptor alpha delta 3*,4,5,6,7*/819 2 isoform antibody
    • Estrogen receptor alpha delta 4 +49 isoform antibody
    • Estrogen receptor alpha delta 4*,5,6,7*/654 isoform antibody
    • Estrogen receptor alpha delta 4*,5,6,7,8*/901 isoform antibody
    • Estrogen receptor alpha E1 E2 1 2 antibody
    • Estrogen receptor alpha E1 N2 E2 1 2 antibody
    • Estrogen receptor antibody
    • ESTRR antibody
    • NR3A1 antibody
    • Nuclear receptor subfamily 3 group A member 1 antibody
    see all


  • Immunohistochemical staining of human breast carcinoma labelling Estrogen Receptor alpha [SP1] with ab93739 at 1:200 dilution.

  • Staining of human breast carcinoma using ab27595
  • Anti-Estrogen Receptor alpha antibody [SP1], prediluted (ab27595) at 1/25 dilution + lysate prepared from MCF7 cells

    Image produced using the diluted version of this antibody, ab16660.


This product has been referenced in:
  • Li Y  et al. Genetic targeting of Purkinje fibres by Sema3a-CreERT2. Sci Rep 8:2382 (2018). IHC-FoFr ; Mouse . Read more (PubMed: 29403069) »
  • Gu J  et al. Knockdown of HIF-1a by siRNA-expressing plasmid delivered by attenuated Salmonella enhances the antitumor effects of cisplatin on prostate cancer. Sci Rep 7:7546 (2017). Read more (PubMed: 28790395) »

See all 14 Publications for this product


ab27595 is specific for ER-alpha. We do not have any experimental data regarding the cross-reactivity of ab27595 with ER-beta. But there is no overlap between the immunogen for ab27595 and the ER-beta protein (Q92731), so theoretically it should...

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Thank you for your inquiry.

Since the working dilutions of antibodies are not a fixed value but have to optimised by the endusers themselves according to experimental condition, I cannot give you absolute numbers. Even when the pre-diluted an...

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Thank you for contacting us.

As antigen retrieval solution, citrate buffer pH 6.0 was used.
Please see below for the detailed protocol:

Pretreatment (Antigen retrieval):

Step 1. Heat slides in citrate buffer in micr...

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Thank you for your inquiry. No, based on immunogen sequence ab27595 will not recognize a sequence between aa 302-553. I am sorry that this information could not be more helpful. Please contact me with any other questions.