Anti-Estrogen Receptor alpha抗体[6F11] (ab93021)

概述

  • 产品名称
    Anti-Estrogen Receptor alpha抗体[6F11]
    参阅全部 Estrogen Receptor alpha 一抗
  • 描述
    小鼠单克隆抗体[6F11] to Estrogen Receptor alpha
  • 经测试应用
    适用于: IHC-P, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant full length protein corresponding to Human Estrogen Receptor alpha.

  • 阳性对照
    • Breast carcinoma

性能

应用

Our Abpromise guarantee covers the use of ab93021 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/20 - 1/80.
WB Use at an assay dependent concentration. Predicted molecular weight: 66 kDa. PubMed: 25212826

靶标

  • 功能
    Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Can activate the transcriptional activity of TFF1.
  • 序列相似性
    Belongs to the nuclear hormone receptor family. NR3 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • 结构域
    Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
  • 翻译后修饰
    Phosphorylated by cyclin A/CDK2. Phosphorylation probably enhances transcriptional activity.
    Glycosylated; contains N-acetylglucosamine, probably O-linked.
    Ubiquitinated. Deubiquitinated by OTUB1.
    Dimethylated by PRMT1 at Arg-260. The methylation may favor cytoplasmic localization.
    Palmitoylated (isoform 3). Not biotinylated (isoform 3).
  • 细胞定位
    Nucleus. Cytoplasm. Cell membrane. A minor fraction is associated with the inner membrane and Nucleus. Cytoplasm. Cell membrane. Associated with the inner membrane via palmitoylation.
  • Information by UniProt
  • 数据库链接
  • 别名
    • DKFZp686N23123 antibody
    • ER alpha antibody
    • ER antibody
    • ER-alpha antibody
    • Era antibody
    • ESR antibody
    • ESR1 antibody
    • ESR1_HUMAN antibody
    • ESRA antibody
    • Estradiol receptor antibody
    • Estrogen nuclear receptor alpha antibody
    • Estrogen receptor 1 antibody
    • Estrogen receptor alpha 3*,4,5,6,7*/822 isoform antibody
    • Estrogen receptor alpha antibody
    • Estrogen receptor alpha delta 3*,4,5,6,7*,8*/941 isoform antibody
    • Estrogen receptor alpha delta 3*,4,5,6,7*/819 2 isoform antibody
    • Estrogen receptor alpha delta 4 +49 isoform antibody
    • Estrogen receptor alpha delta 4*,5,6,7*/654 isoform antibody
    • Estrogen receptor alpha delta 4*,5,6,7,8*/901 isoform antibody
    • Estrogen receptor alpha E1 E2 1 2 antibody
    • Estrogen receptor alpha E1 N2 E2 1 2 antibody
    • Estrogen receptor antibody
    • ESTRR antibody
    • NR3A1 antibody
    • Nuclear receptor subfamily 3 group A member 1 antibody
    see all

图片

  • Formalin-fixed, paraffin-embedded human breast carcinoma tissue stained for Estrogen Receptor alpha using ab93021 at 1/20 dilution in immunohistochemical analysis.

  • ab93021 staining Estrogen Receptor alpha in formalin-fixed, paraffin-embedded Human breast carcinoma tissue.
    Note: the nuclei of the positive cells stain very strongly, without any staining in the cytoplasm.

  • ab93021 staining Estrogen Receptor alpha in formalin-fixed, paraffin-embedded Human breast carcinoma tissue by Immunohistochemistry.

文献

This product has been referenced in:
  • Fu X  et al. Overcoming endocrine resistance due to reduced PTEN levels in estrogen receptor-positive breast cancer by co-targeting mammalian target of rapamycin, protein kinase B, or mitogen-activated protein kinase kinase. Breast Cancer Res 16:430 (2014). WB ; Human . Read more (PubMed: 25212826) »
  • Lit LC  et al. LATS2 is a modulator of estrogen receptor alpha. Anticancer Res 33:53-63 (2013). WB, IHC-P ; Human . Read more (PubMed: 23267128) »

See all 10 Publications for this product

客户评价及客户问答

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Brain)
Permeabilization
Yes - 0.5% Tween-20
Specification
Brain
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5%
Fixative
Paraformaldehyde
Username

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Verified customer

提交于 Aug 02 2017

Suggested dilution range in IHC-P is 1:20 to 1:80 and this antibody requires antigen retrieval with citrate buffer pH 6.0. 

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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