Anti-Estrogen-induced gene 121 protein抗体(ab81247)

概述

  • 产品名称Anti-Estrogen-induced gene 121 protein抗体
    参阅全部 Estrogen-induced gene 121 protein 一抗
  • 描述
    兔多克隆抗体to Estrogen-induced gene 121 protein
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog
  • 免疫原

    Synthetic peptide corresponding to a region within N terminal amino acids 108-157 (CKPCAEGRYS LGTGIRFDEW DELPHGFASL SANMELDDSA AESTGNCTSS) of Human Estrogen-induced gene 121 protein (NP_065826)

  • 阳性对照
    • HeLa cell lysate

性能

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应用

Our Abpromise guarantee covers the use of ab81247 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 111 kDa (predicted molecular weight: 111 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/312500.

靶标

Anti-Estrogen-induced gene 121 protein antibody 图像

  • Anti-Estrogen-induced gene 121 protein antibody (ab81247) at 1 µg/ml (in 5% skim milk / PBS buffer) + HeLa cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 111 kDa
    Observed band size : 111 kDa
    Additional bands at : 80 kDa. We are unsure as to the identity of these extra bands.

Anti-Estrogen-induced gene 121 protein antibody (ab81247)参考文献

ab81247 has not yet been referenced specifically in any publications.

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