概述

  • 产品名称Estriol ELISA试剂盒
    参阅全部 Estriol 试剂盒
  • 检测方法Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Control Sera 32 < 9.7%
    批次间
    样品 n Mean SD CV%
    Control Sera 36 < 10%
  • 测试
    1 x 96 test
  • 样品类型
    Serum, Plasma
  • 检测类型Competitive
  • 灵敏度
    0.22 ng/ml
  • 范围
    2 ng/ml - 200 ng/ml
  • 复检

    94.88 %

  • 实验步骤Multiple steps standard assay
  • 产品概述

    Abcam’s Estriol in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Estriol in serum and plasma.

     

    A 96-well plate has been precoated with anti-Estriol. Samples and the Estriol-HRP conjugate are added to the wells, where Estriol in the sample competes with the added Estriol-HRP for antibody binding. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is inversely proportional to the amount of Estriol in the sample and the intensity is measured at 450 nm.

  • 经测试应用适用于: Competitive ELISAmore details
  • 平台Microplate

性能

  • 别名
    • Oestriol

应用

Our Abpromise guarantee covers the use of ab108675 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Competitive ELISA Use at an assay dependent concentration.

实验方案

Estriol ELISA Kit (ab108675)参考文献

ab108675 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"