概述

  • 产品名称
  • 描述
    兔多克隆抗体to ESM1
  • 经测试应用
    适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Full length protein, corresponding to amino acids 1-184 of Human ESM1 (NP_008967.1).

  • 阳性对照
    • Human kidney tissue lysate; ESM1 transfected 293T cell line lysate

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    Preservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • 纯度
    Protein A purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab103590 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Predicted molecular weight: 20 kDa.
ICC/IF Use at an assay dependent concentration.

靶标

  • 功能
    May have potent implications in lung endothelial cell-leukocyte interactions.
  • 组织特异性
    Expressed in lung, on the vascular capillary network within alveolar walls, and also at lower level in kidney.
  • 序列相似性
    Contains 1 IGFBP N-terminal domain.
  • 翻译后修饰
    May contain intrachain disulfide bonds.
  • 细胞定位
    Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Endocan antibody
    • Endothelial cell specific molecule 1 antibody
    • Endothelial cell-specific molecule 1 antibody
    • ESM 1 antibody
    • ESM 1 secretory protein antibody
    • ESM-1 antibody
    • Esm1 antibody
    • ESM1 secretory protein antibody
    • ESM1_HUMAN antibody
    see all

Anti-ESM1 antibody 图像

  • Anti-ESM1 antibody (ab103590) at 1/500 dilution + Human kidney tissue lysate at 50 µg

    Predicted band size : 20 kDa
  • All lanes : Anti-ESM1 antibody (ab103590) at 1/500 dilution

    Lane 1 : ESM1 transfected 293T cell line lysate
    Lane 2 : Non-transfected 293T cell line lysate

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 20 kDa
  • ab103590 staining ESM1 in the Human HUVEC cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with Triton X-100 0.1% and blocked with 10% serum for 60 minutes at 24°C. Samples were incubated with primary antibody (1/100) for 16 hours at 4°C. An Alexa Fluor®488-conjugated Goat anti-rabbit monoclonal (1/500) was used as the secondary antibody.

    See Abreview

Anti-ESM1 antibody (ab103590)参考文献

This product has been referenced in:
  • Chen CM  et al. Loss of endothelial cell-specific molecule 1 promotes the tumorigenicity and metastasis of prostate cancer cells through regulation of the TIMP-1/MMP-9 expression. Oncotarget 8:13886-13897 (2017). Read more (PubMed: 28108731) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (NIH3T3)
Specification
NIH3T3
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% Triton
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C
Username

Abcam user community

Verified customer

提交于 Mar 26 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HUVEC)
Specification
HUVEC
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% Triton
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
Username

Abcam user community

Verified customer

提交于 Feb 28 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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