Anti-ESCO2抗体(ab107277)

概述

  • 产品名称
  • 描述
    兔多克隆抗体to ESCO2
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    KLH conjugated synthetic peptide corresponding to a region within internal sequence amino acids 135-165 of Human ESCO2 (NP_001017420.1).

  • 阳性对照
    • Mouse stomach tissue lysate

性能

应用

Our Abpromise guarantee covers the use of ab107277 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/100 - 1/500. Predicted molecular weight: 68 kDa.

靶标

  • 相关性
    ESCO2 is an acetyltransferase required for the establishment of sister chromatid cohesion, and couples the processes of cohesion and DNA replication to ensure that only sister chromatids become paired together. In contrast to the structural cohesins, the deposition and establishment factors are required only during S phase. Defects in ESCO2 are the cause of Roberts syndrome (RBS), a rare autosomal recessive disorder characterized by pre- and postnatal growth retardation, microcephaly, bilateral cleft lip and palate, and mesomelic symmetric limb reduction. Severely affected infants may be stillborn or die shortly after birth. RBS chromosomes have a lack of cohesion involving the heterochromatic C-banding regions around centromeres and the distal portion of the long arm of the Y chromosome (known as premature centromere separation, heterochromatin repulsion or puffing, or RS effect). Defects in ESCO2 are also the cause of SC phocomelia syndrome, also known as SC pseudothalidomide syndrome. SC phocomelia syndrome has a milder phenotype than RBS, with a lesser degree of symmetric limb reduction and additionally includes flexion contractures of various joints, midfacial hemangioma, hypoplastic cartilage of ears and nose, scant silvery-blond hair, and cloudy corneae. Although microcephaly is present, mental retardation may be mild and survival into adulthood is common.
  • 细胞定位
    Nuclear
  • 数据库链接
  • 别名
    • CTF7, S. CEREVISIAE, HOMOLOG OF, 2 antibody
    • ECO1 homolog 2 antibody
    • ECO1, S. CEREVISIAE, HOMOLOG OF, 2 antibody
    • EFO2 antibody
    • ESO1, S. POMBE, HOMOLOG OF, 2 antibody
    • ESTABLISHMENT FACTOR ORTHOLOG 2; EFO2 antibody
    • Establishment of cohesion 1 homolog 2 antibody
    • Establishment of cohesion 1 homolog 2 (S. cerevisiae) antibody
    • N acetyltransferase ESCO2 antibody
    • RBS antibody
    • Roberts syndrome antibody
    see all

图片

  • Anti-ESCO2 antibody (ab107277) at 1/100 dilution + Mouse stomach tissue lysate at 35 µg

    Predicted band size : 68 kDa

实验方案

文献

ab107277 has not yet been referenced specifically in any publications.

客户评价及客户问答

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Permeabilization
Yes - 0.5% TritonX100 in PBS
Specification
HeLa
Fixative
Paraformaldehyde
Username

Dr. Kirk Mcmanus

Verified customer

提交于 Aug 11 2015

Application
Western blot
Sample
Human Cell lysate - whole cell (Human osteosarcoma U2OS cells)
Loading amount
100 µg
Specification
Human osteosarcoma U2OS cells
Gel Running Conditions
Reduced Denaturing (4-18% gel)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Jun 13 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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