Anti-ESAM抗体(ab74777)

概述

  • 产品名称Anti-ESAM抗体
    参阅全部 ESAM 一抗
  • 描述
    兔多克隆抗体to ESAM
  • 经测试应用适用于: ICC/IF, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Cynomolgus Monkey
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Human ESAM.

    (Peptide available as ab101730.)

  • 阳性对照
    • This antibody gave a positive signal in the following lysates: Human Heart tissue; Human Liver tissue; Human Kidney tissue; Human Lung tissue; HepG2 whole cell; HEK293 whole cell.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab74777 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 41 kDa).

靶标

  • 功能Can mediate aggregation most likely through a homophilic molecular interaction.
  • 组织特异性Highly expressed in endothelial cells.
  • 序列相似性Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • 细胞定位Cell junction > adherens junction. Cell junction > tight junction. Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 2310008D05Rik antibody
    • Endothelial cell adhesion molecule antibody
    • Endothelial cell selective adhesion molecule antibody
    • Endothelial cell-selective adhesion molecule antibody
    • Esam antibody
    • ESAM_HUMAN antibody
    • HUEL (C4orf1) interacting protein antibody
    • LP4791 protein antibody
    • W117m antibody
    see all

Anti-ESAM antibody 图像

  • All lanes : Anti-ESAM antibody (ab74777) at 1 µg/ml

    Lane 1 : Human heart tissue lysate - total protein (ab29431)
    Lane 2 : Human liver tissue lysate - total protein (ab29889)
    Lane 3 : Human kidney tissue lysate - total protein (ab30203)
    Lane 4 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 5 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 6 : Lung (Human) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Annexin A2 overexpression lysate (ab94080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 41 kDa
    Observed band size : 37,41 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 100 kDa,15 kDa,250 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 3 minutes
  • ICC/IF image of ab74777 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab74777, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) HepG2 cells at 5µg/ml, and in 100% methanol fixed (5 min) HepG2 and MCF7 cells at 5µg/ml.

Anti-ESAM antibody (ab74777)参考文献

ab74777 has not yet been referenced specifically in any publications.

Product Wall

Sorry I was not able to take your email last week. I was not in the office. I am very sorry that the two anti-ESAM antibodies did not show the staining we had hoped for, it may be that as you say that the epitope presented by the protein (even thou...

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Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Colon tumour)
Specification Colon tumour
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate pH6.0
Permeabilization No
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C
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Abcam user community

Verified customer

提交于 Nov 08 2011

Thank you for contacting us. We currently have two antibodies directed towards human EMSA, ab74777 and ab73988. Both are rabbit polyclonals. ab74777 like the antibody you have previously used is directed against the extracellular domain of ESAM...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"