概述

  • 产品名称Anti-ERp57抗体
    参阅全部 ERp57 一抗
  • 描述
    兔多克隆抗体to ERp57
  • 经测试应用适用于: Flow Cyt, IHC-P, ICC/IF, WB, ICC, IPmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    His-tagged E. coli expressed recombinant ERp57.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 纯度Whole antiserum
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab10287 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use at an assay dependent concentration. PubMed: 23762310

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

IHC-P Use at an assay dependent concentration. PubMed: 20731657
ICC/IF Use at an assay dependent concentration.
WB 1/1000 - 1/3000. Detects a band of approximately 57 kDa (predicted molecular weight: 61 kDa).
ICC 1/100.
IP Use at an assay dependent concentration.

靶标

  • 组织特异性Detected in the flagellum and head region of spermatozoa (at protein level).
  • 序列相似性Belongs to the protein disulfide isomerase family.
    Contains 2 thioredoxin domains.
  • 细胞定位Endoplasmic reticulum lumen. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 58 kDa glucose regulated protein antibody
    • 58 kDa glucose-regulated protein antibody
    • 58 kDa microsomal protein antibody
    • Disulfide isomerase ER 60 antibody
    • Disulfide isomerase ER-60 antibody
    • Endoplasmic reticulum resident protein 57 antibody
    • Endoplasmic reticulum resident protein 60 antibody
    • ER p57 antibody
    • ER protein 57 antibody
    • ER protein 60 antibody
    • ERp 57 antibody
    • ERp57 antibody
    • ERp60 antibody
    • ERp61 antibody
    • Glucose Regulated Protein 58 Kd antibody
    • GRP 57 antibody
    • GRP 58 antibody
    • GRP57 antibody
    • HsT17083 antibody
    • p58 antibody
    • PDIA 3 antibody
    • PDIA3 antibody
    • PDIA3_HUMAN antibody
    • Phospholipase C alpha antibody
    • PI PLC antibody
    • Protein disulfide isomerase A3 antibody
    • Protein disulfide isomerase family A member 3 antibody
    • Protein disulfide-isomerase A3 antibody
    see all

Anti-ERp57 antibody 图像

  • All lanes : Anti-ERp57 antibody (ab10287) at 1/1000 dilution

    Lane 1 : Mouse embryonic cells
    Lane 2 : Calreticulin deficient mouse embryomic cells


    Predicted band size : 61 kDa
    Observed band size : 57 kDa (why is the actual band size different from the predicted?)

    Western blot using ab10287 at 1/1000.

    Lane 1 – Mouse Embryonic Fibroblasts.
    Lane 2 – Calreticulin deficient Mouse Embryonic Fibroblasts.

  • Double staining of mouse embryonic fibroblasts (top) and calreticulin deficient fibroblasts (bottom) with rabbit-anti-ERp57 antibodies (1:100;  upper and lower left panel) and FITC-ConA (upper and lower right panel).
  • ab10287 at 1/100 staining human HeLa cells by ICC/IF. The cells were premeabilized with digitonin before being blocked and then stained with the antibody for 1 hour. An Alexa-Fluor ® conjugated goat anti rabbit antibody was used as the secondary.

    See Abreview

  • ab10287 (1/2000) staining ERp57 in human colon using an automated system (DAKO Autostainer Plus). Using this protocol there is strong cytoplasmic staining.
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer citrate pH 6.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
  • ERp57 was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to ERp57 (ab10287) and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
    The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab10287.
    Secondary: Antibody to Rabbit IgG light chain (HRP) (ab99697).
    Band: 61kDa: ERp57

Anti-ERp57 antibody (ab10287)参考文献

This product has been referenced in:
  • Kodavanti PR  et al. Developmental exposure to a commercial PBDE mixture: effects on protein networks in the cerebellum and hippocampus of rats. Environ Health Perspect 123:428-36 (2015). WB ; Rat . Read more (PubMed: 25616259) »
  • Leitman J  et al. Herp coordinates compartmentalization and recruitment of HRD1 and misfolded proteins for ERAD. Mol Biol Cell 25:1050-60 (2014). Human . Read more (PubMed: 24478453) »

See all 14 Publications for this product

Product Wall

Application Western blot
Loading amount 15 µg
Gel Running Conditions Non-reduced Denaturing (12.5%)
Sample Mouse Tissue lysate - other (white fat tissue)
Specification white fat tissue
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 Jan 22 2014

Thank you for your enquiry.

As the datasheet indicates this antibody is whole antiserum and not affinity purified therefore the concentration is not determined. Generally, the IgG concentration for serum antibody is 1-10 mg/ml.

You ...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Formaldehyde
Permeabilization Yes - Digitonin
Blocking step BSA as blocking agent for 20 minute(s) · Concentration: 4% · Temperature: 22°C
Username

Abcam user community

Verified customer

提交于 Sep 14 2007

Thank you for your enquiry and your interest in our products. We would like to confirm that this antibody was raised in rabbit against mouse recombinant ERp57. It does cross-react with mouse but other species have not tested yet.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"