概述

  • 产品名称Anti-ERp29抗体
    参阅全部 ERp29 一抗
  • 描述
    山羊多克隆抗体to ERp29
  • 特异性ab77545 is expected to recognize isoform 1 (NP_006808.1) only.
  • 经测试应用适用于: ELISA, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Sheep, Horse, Chicken, Cow, Dog, Pig, Chimpanzee, Rhesus monkey
  • 免疫原

    Synthetic peptide:

    EKNKMSDGKKEELQ

    , corresponding to C terminal amino acids 229-242 of Human ERp29 (NP_006808.1).

  • 阳性对照
    • Human Liver lysate.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris saline, pH 7.3
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明ab77545 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunising peptide.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab77545 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA
WB
  • 应用说明Peptide ELISA: Antibody detection limit dilution 1/2000.
    WB: Use at a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 相关性Proper protein folding and post-translational modifications are essential for secretory protein export out of the endoplasmic reticulum. This task is accomplished by chaperone proteins such as protein disulfide isomerase (PDI), GRP94, and BiP. A recently characterized protein, designated ERp29, is closely related to these chaperone proteins and appears to be upregulated during ER stress conditions. ERp29 is a soluble 259-residue protein that is localized to the lumen of the endoplasmic reticulum in all mammalian cells. Research has shown that there are two primary domains within ERp29. The first is the C-terminal region that is a novel, all helical, fold that is most likely involved with ERp29 retention to the ER. The second is the N-terminal region that resembles that of PDI’s thioredoxin module. The protein shows sequence similarity to the protein disulfide isomerase family. However, it lacks the thioredoxin motif characteristic of this family, suggesting that this protein does not function as a disulfide isomerase. The protein dimerizes and is thought to play a role in the processing of secretory proteins within the ER.
    • 细胞定位Endoplasmic reticulum, Cell surface.
    • 数据库链接
    • 别名
      • C12orf8 antibody
      • Chromosome 12 open reading frame 8 antibody
      • Endoplasmic reticulum lumenal protein ERp28 antibody
      • Endoplasmic reticulum protein 29 antibody
      • Endoplasmic reticulum protein 29 isoform 1 antibody
      • Endoplasmic reticulum protein ERp29 antibody
      • Endoplasmic reticulum resident protein 28 antibody
      • Endoplasmic reticulum resident protein 29 antibody
      • Endoplasmic reticulum resident protein 31 antibody
      • ERp28 antibody
      • ERP29_HUMAN antibody
      • ERp31 antibody
      • PDI-DB antibody
      • PDIA9 antibody
      • Protein disulfide isomerase family A member 9 antibody
      see all

    Anti-ERp29 antibody 图像

    • Anti-ERp29 antibody (ab77545) at 0.1 µg/ml + Human Liver lysate (in RIPA buffer) at 35 µg
      Developed using the ECL technique

      Predicted band size : 29 kDa
      Observed band size : 29 kDa

    Anti-ERp29 antibody (ab77545)参考文献

    ab77545 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab77545.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"