概述

  • 产品名称Anti-ERp29抗体
    参阅全部 ERp29 一抗
  • 描述
    兔多克隆抗体to ERp29
  • 经测试应用适用于: Flow Cyt, IHC-Fr, ICC, WB, IP, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Guinea pig, Hamster, Cow, Dog, Human, Non Human Primates
  • 免疫原

    Recombinant full length protein (His-tag) corresponding to Rat ERp29.

  • 阳性对照
    • Western blot: rat liver homogenate. Immunofluorescence: rat thyrocytes.

性能

应用

Our Abpromise guarantee covers the use of ab11420 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt 1/500. PubMed: 17451556

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

IHC-Fr Use at an assay dependent concentration. PubMed: 17652758
ICC Use at an assay dependent concentration.
WB 1/2500. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa).

This antibody detects an 29 kDa protein representing ERp29 in rat liver homogenate.

IP 1/500.
ICC/IF 1/500.

Immunofluorescence data demonstrates that ERp29 is localized to the ER of rat thyrocytes using ab11420.

靶标

  • 相关性Proper protein folding and post-translational modifications are essential for secretory protein export out of the endoplasmic reticulum. This task is accomplished by chaperone proteins such as protein disulfide isomerase (PDI), GRP94, and BiP. A recently characterized protein, designated ERp29, is closely related to these chaperone proteins and appears to be upregulated during ER stress conditions. ERp29 is a soluble 259-residue protein that is localized to the lumen of the endoplasmic reticulum in all mammalian cells. Research has shown that there are two primary domains within ERp29. The first is the C-terminal region that is a novel, all helical, fold that is most likely involved with ERp29 retention to the ER. The second is the N-terminal region that resembles that of PDI’s thioredoxin module. The protein shows sequence similarity to the protein disulfide isomerase family. However, it lacks the thioredoxin motif characteristic of this family, suggesting that this protein does not function as a disulfide isomerase. The protein dimerizes and is thought to play a role in the processing of secretory proteins within the ER.
  • 细胞定位Endoplasmic reticulum, Cell surface.
  • 数据库链接
  • 别名
    • C12orf8 antibody
    • Chromosome 12 open reading frame 8 antibody
    • Endoplasmic reticulum lumenal protein ERp28 antibody
    • Endoplasmic reticulum protein 29 antibody
    • Endoplasmic reticulum protein 29 isoform 1 antibody
    • Endoplasmic reticulum protein ERp29 antibody
    • Endoplasmic reticulum resident protein 28 antibody
    • Endoplasmic reticulum resident protein 29 antibody
    • Endoplasmic reticulum resident protein 31 antibody
    • ERp28 antibody
    • ERP29_HUMAN antibody
    • ERp31 antibody
    • PDI-DB antibody
    • PDIA9 antibody
    • Protein disulfide isomerase family A member 9 antibody
    see all

Anti-ERp29 antibody 图像

  • ICC/IF image of ab11420 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab11420, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Immunocytochemistry/Immunofluorescence analysis of HMVEC cells labeling ERp29 with ab11420.

  • Immunocytochemistry/Immunofluorescence analysis of NS-1 cells labeling ERp29 with ab11420.

  • Immunocytochemistry/Immunofluorescence analysis of p19 cells labeling ERp29 with ab11420.



  • Predicted band size : 29 kDa
    Western blot of ERp29 in HEK cell lysate using ab11420.
  • Immunocytochemistry/Immunofluorescence analysis of rat thyrocytes labeling ERp29 with ab11420.

Anti-ERp29 antibody (ab11420)参考文献

This product has been referenced in:
  • Song J  et al. Native low density lipoprotein promotes lipid raft formation in macrophages. Mol Med Rep 13:2087-93 (2016). Mouse . Read more (PubMed: 26781977) »
  • Holbrook LM  et al. Platelets release novel thiol isomerase enzymes which are recruited to the cell surface following activation. Br J Haematol 148:627-37 (2010). WB ; Human . Read more (PubMed: 19995400) »

See all 8 Publications for this product

Product Wall

Application Western blot
Sample Mouse Tissue lysate - whole (Retinas)
Gel Running Conditions Non-reduced Denaturing (TGX gel)
Loading amount 10 µg
Specification Retinas
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Dr. Minzhong Yu

Verified customer

提交于 May 09 2016

Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Retina)
Permeabilization Yes - Triton X-100
Specification Retina
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 21°C
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 May 02 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (Liver)
Loading amount 20 µg
Specification Liver
Gel Running Conditions Reduced Non-Denaturing (Native) (4-20%)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 24°C
Username

Abcam user community

Verified customer

提交于 Nov 29 2012

Thank you for your telephone call yesterday afternoon.

I am pleased to provide some information regarding suitable secondaries for the primary antibodies you have selected:

ab48394 LC3B antibody, ab24170 LAMP1antibody andab11420 E...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Cell lysate - whole cell (PC12 whole cell lysate)
Loading amount 25 µg
Specification PC12 whole cell lysate
Gel Running Conditions Reduced Denaturing (10% polyacrylamide SDS gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.2% · Temperature: 22°C
Username

Abcam user community

Verified customer

提交于 Oct 08 2008

Thank you for your enquiry. Unfortunately the IgG concentration for ab11420 has not been determined. However, I would estimate that the concentration is between 1-2 mg/ml. I apologize that I could not be of more assistance.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"