The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. (Heat mediated/Sodium citrate pH 6 was the method that was used for antigen retrieval in the IHC image below).
Immunohistochemistry analysis in formalin fixed and paraffin embedded mouse testis tissue using ab103704 at 1/50 dilution, followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of ab103704 for immunohistochemistry. Clinical relevance has not been evaluated.