The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
应用说明WB: Use at a concentration of 1-5 µg/ml.
Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
功能Essential oxidoreductase that oxidizes proteins in the endoplasmic reticulum to produce disulfide bonds. Acts by oxidizing directly P4HB/PDI isomerase through a direct disulfide exchange. Does not act as a direct oxidant of folding substrate, but relies on P4HB/PDI to transfer oxidizing equivalent. Associates with ERP44 but not with GRP54, demonstrating that it does not oxidize all PDI related proteins and can discriminate between PDI and related proteins. Its reoxidation probably involves electron transfer to molecular oxygen via FAD. Acts independently of glutathione. May be responsible for a significant proportion of reactive oxygen species (ROS) in the cell, thereby being a source of oxidative stress. Required for the folding of immunoglobulin proteins. Responsible for the release of the unfolded cholera toxin from reduced P4HB/PDI in case of infection by V.cholerae, thereby playing a role in retrotranslocation of the toxin.
组织特异性Widely expressed at low level. Expressed at high level in upper digestive tract. Highly expressed in esophagus. Weakly expressed in stomach and duodenum.
序列相似性Belongs to the EROs family.
翻译后修饰N-glycosylated. The Cys-94/Cys-99 and Cys-394/Cys-397 disulfide bonds constitute the redox-active center. The Cys-94/Cys-99 disulfide bond may accept electron from P4HB and funnel them to the active site disulfide Cys-394/Cys-397.
细胞定位Endoplasmic reticulum membrane. The association with ERP44 is essential for its retention in the endoplasmic reticulum.
ERO1-like, S. cerevisiae, homolog of, alpha antibody
Oxidoreductin 1 Lalpha antibody
Anti-ERO1L antibody 图像
Western blot - ERO1L antibody (ab57177)
Predicted band size : 54 kDa ERO1L antibody (ab57177) at 1ug/lane + HeLa cell lysate at 25ug/lane.
Anti-ERO1L antibody (ab57177)参考文献
This product has been referenced in:
Zhang L et al. Different interaction modes for protein-disulfide isomerase (PDI) as an efficient regulator and a specific substrate of endoplasmic reticulum oxidoreductin-1a (Ero1a). J Biol Chem289:31188-99 (2014).
Read more (PubMed: 25258311) »