概述

  • 产品名称Anti-ERO1L抗体
    参阅全部 ERO1L 一抗
  • 描述
    小鼠单克隆抗体to ERO1L
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant fragment: DISQCGRRDC AVKPCQSDEV PDGIKSASYK YSEEANNLIE ECEQAERLGA VDESLSEETQ KAVLQWTKHD DSSDNFCEAD DIQSPEAEY, corresponding to amino acids 90-179 of Human ERO1L

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: None
    PBS, pH 7.2
  • Concentration information loading...
  • 纯度Protein G purified
  • 克隆单克隆
  • 同种型IgG2b
  • 轻链类型kappa
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab57177 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
  • 应用说明WB: Use at a concentration of 1-5 µg/ml.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Essential oxidoreductase that oxidizes proteins in the endoplasmic reticulum to produce disulfide bonds. Acts by oxidizing directly P4HB/PDI isomerase through a direct disulfide exchange. Does not act as a direct oxidant of folding substrate, but relies on P4HB/PDI to transfer oxidizing equivalent. Associates with ERP44 but not with GRP54, demonstrating that it does not oxidize all PDI related proteins and can discriminate between PDI and related proteins. Its reoxidation probably involves electron transfer to molecular oxygen via FAD. Acts independently of glutathione. May be responsible for a significant proportion of reactive oxygen species (ROS) in the cell, thereby being a source of oxidative stress. Required for the folding of immunoglobulin proteins. Responsible for the release of the unfolded cholera toxin from reduced P4HB/PDI in case of infection by V.cholerae, thereby playing a role in retrotranslocation of the toxin.
    • 组织特异性Widely expressed at low level. Expressed at high level in upper digestive tract. Highly expressed in esophagus. Weakly expressed in stomach and duodenum.
    • 序列相似性Belongs to the EROs family.
    • 翻译后修饰N-glycosylated.
      The Cys-94/Cys-99 and Cys-394/Cys-397 disulfide bonds constitute the redox-active center. The Cys-94/Cys-99 disulfide bond may accept electron from P4HB and funnel them to the active site disulfide Cys-394/Cys-397.
    • 细胞定位Endoplasmic reticulum membrane. The association with ERP44 is essential for its retention in the endoplasmic reticulum.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Endoplasmic oxidoreductin 1 like protein antibody
      • Endoplasmic oxidoreductin-1-like protein antibody
      • Endoplasmic reticulum oxidoreductin 1-like antibody
      • ERO1 alpha antibody
      • ERO1 L antibody
      • ERO1 Lalpha antibody
      • ERO1 like protein alpha antibody
      • ERO1-alpha antibody
      • ERO1-L antibody
      • ERO1-L-alpha antibody
      • ERO1-like (S. cerevisiae) antibody
      • ERO1-like alpha antibody
      • ERO1-like protein alpha antibody
      • ERO1-like, S. cerevisiae, homolog of, alpha antibody
      • ERO1A antibody
      • ERO1A_HUMAN antibody
      • ERO1L antibody
      • ERO1LA antibody
      • Oxidoreductin 1 Lalpha antibody
      • Oxidoreductin-1-L-alpha antibody
      • PRO865 antibody
      • UNQ434 antibody
      see all

    Anti-ERO1L antibody 图像



    • Predicted band size : 54 kDa
      ERO1L antibody (ab57177) at 1ug/lane + HeLa cell lysate at 25ug/lane.

    Anti-ERO1L antibody (ab57177)参考文献

    This product has been referenced in:
    • Zhang L  et al. Different interaction modes for protein-disulfide isomerase (PDI) as an efficient regulator and a specific substrate of endoplasmic reticulum oxidoreductin-1a (Ero1a). J Biol Chem 289:31188-99 (2014). Read more (PubMed: 25258311) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"