Anti-ERN2抗体- C-terminal (ab71348)

概述

  • 产品名称Anti-ERN2抗体- C-terminal
    参阅全部 ERN2 一抗
  • 描述
    兔多克隆抗体to ERN2 - C-terminal
  • 经测试应用适用于: ELISA, WBmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide from the C-terminal region of human ERN2 conjugated to KLH

  • 阳性对照
    • Mouse liver tissue lysate.

性能

应用

Our Abpromise guarantee covers the use of ab71348 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/1000.
WB 1/100 - 1/500. Detects a band of approximately 125 kDa (predicted molecular weight: 102 kDa).

靶标

  • 功能Induces translational repression through 28S ribosomal RNA cleavage in response to ER stress. Pro-apoptotic. Appears to play no role in the unfolded-protein response, unlike closely related proteins.
  • 序列相似性Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.
    Contains 1 KEN domain.
    Contains 1 protein kinase domain.
  • 翻译后修饰Autophosphorylated.
  • 细胞定位Endoplasmic reticulum membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Endoplasmic reticulum to nucleus signaling 2 antibody
    • Endoplasmic reticulum-to-nucleus signaling 2 antibody
    • Endoribonuclease antibody
    • ER to nucleus signalling 2 antibody
    • ERN 2 antibody
    • Ern1 antibody
    • Ern2 antibody
    • ERN2_HUMAN antibody
    • hIRE2p antibody
    • Inositol requiring 1 beta antibody
    • Inositol requiring protein 2 antibody
    • Inositol-requiring protein 2 antibody
    • Ire1 antibody
    • Ire1 beta antibody
    • IRE1 S. cerevisiae homolog of antibody
    • Ire1-beta antibody
    • IRE1b antibody
    • IRE2 antibody
    • MGC124363 antibody
    • MGC124364 antibody
    • Serine/threonine protein kinase/endoribonuclease IRE2 antibody
    see all

Anti-ERN2 antibody - C-terminal 图像

  • Anti-ERN2 antibody - C-terminal (ab71348) at 1/100 dilution + mouse liver tissue lysate at 12.5 µg

    Predicted band size : 102 kDa
    Observed band size : 125 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 32 kDa,45 kDa,48 kDa. We are unsure as to the identity of these extra bands.

Anti-ERN2 antibody - C-terminal (ab71348)参考文献

ab71348 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Human Cell lysate - whole cell (HEK293 cells)
Gel Running Conditions Reduced Denaturing (10%)
Loading amount 20 µg
Treatment 10 and 20 micromolar Cadmium for 6h
Specification HEK293 cells
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Jul 30 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"