兔多克隆抗体to ERM / Etv5
Recombinant fragment, corresponding to a region within amino acids 244-404 of Human ERM/ Etv5 (AAH07333)
A431, Jurkat, Raji cell lysate, PC14 xenograft
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7.0
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration. PubMed: 22933516
1/500 - 1/3000. Predicted molecular weight: 58 kDa.
1/100 - 1/500. Antigen retrieval using heat mediated 10mM Citrate buffer or Tris-EDTA buffer (pH 8.0) is recommended.
Binds to DNA sequences containing the consensus nucleotide core sequence GGAA.
Belongs to the ETS family.
Contains 1 ETS DNA-binding domain.
Information by UniProt
Ets related protein ERM antibody
ETS translocation variant 5 antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERM / Etv5 antibody (ab102010)
ab102010 staining ERM / Etv5 in Mouse colon tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Samples were incubated with primary antibody (1/500).
Immunocytochemistry/ Immunofluorescence - Anti-ERM / Etv5 antibody (ab102010)
ab102010 staining ERM / Etv5 in HeLa cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% paraformaldehyde at room temperature for 15 minutes. Samples were incubated with primary antibody (1/500) and cytoskeleton stained red with Phalloidin.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - ERM / Etv5 antibody (ab102010)
Immunohistochemical analysis of paraffin-embedded PC14 xenograft, using ab102010 at 1/100 dilution.
Western blot - ERM / Etv5 antibody (ab102010)
Anti-ERM / Etv5 antibody (ab102010) at 1/1000 dilution + Raji whole cell lysate at 30 µg
Predicted band size: 58 kDa 7.5% SDS PAGE
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"