概述

  • 产品名称Anti-ERK5抗体[EP791Y]
    参阅全部 ERK5 一抗
  • 描述
    兔单克隆抗体[EP791Y] to ERK5
  • 经测试应用适用于: WB, ICC, Flow Cyt, IPmore details
    不适用于: IHC
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to residues in the C-terminus of Human ERK5.

  • 阳性对照
    • Hela Cell lysate.
  • 常规说明

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

    This product is a recombinant rabbit monoclonal antibody.

性能

应用

Our Abpromise guarantee covers the use of ab40809 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/5000. Detects a band of approximately 115 kDa (predicted molecular weight: 89 kDa).
ICC 1/250 - 1/500.
Flow Cyt 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IP 1/50.
  • 应用说明Is unsuitable for IHC.
  • 靶标

    • 功能Plays a role in various cellular processes such as proliferation, differentiation and cell survival. The upstream activator of MAPK7 is the MAPK kinase MAP2K5. Upon activation, it translocates to the nucleus and phosphorylates various downstream targets including MEF2C. EGF activates MAPK7 through a Ras-independent and MAP2K5-dependent pathway. May have a role in muscle cell differentiation. May be important for endothelial function and maintenance of blood vessel integrity. MAP2K5 and MAPK7 interact specifically with one another and not with MEK1/ERK1 or MEK2/ERK2 pathways.
    • 组织特异性Expressed in many adult tissues. Abundant in heart, placenta, lung, kidney and skeletal muscle. Not detectable in liver.
    • 序列相似性Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
      Contains 1 protein kinase domain.
    • 结构域The second proline-rich region may interact with actin targeting the kinase to a specific location in the cell.
      The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
    • 翻译后修饰Dually phosphorylated on Thr-219 and Tyr-221, which activates the enzyme (By similarity). Autophosphorylated in vitro on threonine and tyrosine residues when the C-terminal part of the kinase, which could have a regulatory role, is absent.
    • 细胞定位Cytoplasm. Nucleus. Translocates to the nucleus upon activation.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Big MAP kinase 1 antibody
      • BMK 1 antibody
      • BMK 1 kinase antibody
      • BMK-1 antibody
      • BMK1 antibody
      • BMK1 Kinase antibody
      • EC 2.7.11.24 antibody
      • ERK 4 antibody
      • ERK 5 antibody
      • ERK-5 antibody
      • ERK4 antibody
      • ERK5 antibody
      • Extracellular signal regulated kinase 5 antibody
      • Extracellular signal-regulated kinase 5 antibody
      • MAP kinase 7 antibody
      • MAPK 7 antibody
      • MAPK7 antibody
      • Mitogen activated protein kinase 7 antibody
      • Mitogen-activated protein kinase 7 antibody
      • MK07_HUMAN antibody
      • OTTHUMP00000065906 antibody
      • OTTHUMP00000065907 antibody
      • PRKM 7 antibody
      • PRKM7 antibody
      • PROTEIN KINASE, MITOGEN-ACTIVATED, 7 antibody
      see all

    Anti-ERK5 antibody [EP791Y] 图像

    • Overlay histogram showing A549 cells stained with ab40809 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab40809, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
    • Anti-ERK5 antibody [EP791Y] (ab40809) at 1/5000 dilution + Hela cell lysate at 10 µg

      Predicted band size : 89 kDa
      Observed band size : 115 kDa (why is the actual band size different from the predicted?)
      The predicted weight of 89 kDa is for the precursor version of human ERK5 protein. However, ab40809 detects endogenous levels of total Erk5 protein which appears around 115 kDa in SDS PAGE

    Anti-ERK5 antibody [EP791Y] (ab40809)参考文献

    This product has been referenced in:
    • Li L  et al. Thymic Stromal Lymphopoietin Promotes Fibrosis and Activates Mitogen-Activated Protein Kinases in MRC-5 Cells. Med Sci Monit 22:2357-62 (2016). WB ; Human . Read more (PubMed: 27385084) »
    • Yoshizuka N  et al. A novel function of p38-regulated/activated kinase in endothelial cell migration and tumor angiogenesis. Mol Cell Biol 32:606-18 (2012). Read more (PubMed: 22124154) »

    See all 2 Publications for this product

    Product Wall

    Following up my previous message, the lab confirmed that ab40809 was negative for IHC on formalin -fixed tissue. However, it was positive for ICC and flow cytometry on PFA-fixed samples. These application use relatively short fixation times compared to...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"