Anti-ERK2抗体[6F8] (ab124362)

概述

  • 产品名称Anti-ERK2抗体[6F8]
    参阅全部 ERK2 一抗
  • 描述
    小鼠单克隆抗体[6F8] to ERK2
  • 经测试应用适用于: WB, IP, IHC-P, Flow Cytmore details
  • 种属反应性
    与反应: Rat, Dog, Human, Monkey
  • 免疫原

    Full length protein of Human ERK2 expressed in 293T cells transfected with expression vector.

  • 阳性对照
    • ERK2-transfected HEK293T cells and lysate, HeLa, HT29, A549, COS7, Jurkat, MDCK, PC12, and MCF7 cell extracts, Adenocarcinoma of Human breast and Adenocarcinoma of Human ovary tissues,

性能

应用

Our Abpromise guarantee covers the use of ab124362 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/2000.
IP Use at 2-0.5 µg/mg of lysate.
IHC-P 1/50.
Flow Cyt 1/100. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

靶标

  • 功能Involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1 (By similarity). Phosphorylates heat shock factor protein 4 (HSF4) and ARHGEF2.
    Acts as a transcriptional repressor. Binds to a [GC]AAA[GC] consensus sequence. Repress the expression of interferon gamma-induced genes. Seems to bind to the promoter of CCL5, DMP1, IFIH1, IFITM1, IRF7, IRF9, LAMP3, OAS1, OAS2, OAS3 and STAT1. Transcriptional activity is independent of kinase activity.
  • 序列相似性Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
    Contains 1 protein kinase domain.
  • 结构域The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
  • 翻译后修饰Dually phosphorylated on Thr-185 and Tyr-187, which activates the enzyme. Dephosphorylated by PTPRJ at Tyr-187.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ERK 2 antibody
    • ERK antibody
    • ERK-2 antibody
    • ERT1 antibody
    • Extracellular Signal Regulated Kinase 2 antibody
    • Extracellular signal-regulated kinase 2 antibody
    • MAP kinase 1 antibody
    • MAP kinase 2 antibody
    • MAP kinase isoform p42 antibody
    • MAPK 1 antibody
    • MAPK 2 antibody
    • Mapk1 antibody
    • MAPK2 antibody
    • Mitogen-activated protein kinase 1 antibody
    • Mitogen-activated protein kinase 2 antibody
    • MK01_HUMAN antibody
    • P38 antibody
    • P40 antibody
    • P41 antibody
    • p42-MAPK antibody
    • P42MAPK antibody
    • PRKM1 antibody
    • PRKM2 antibody
    • protein kinase, mitogen-activated, 1 antibody
    • protein kinase, mitogen-activated, 2 antibody
    • protein tyrosine kinase ERK2 antibody
    see all

Anti-ERK2 antibody [6F8] 图像

  • All lanes : Anti-ERK2 antibody [6F8] (ab124362) at 1/1000 dilution

    Lane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY control
    Lane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY ERK2

    Lysates/proteins at 5 µg per lane.
    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC204703)
  • All lanes : Anti-ERK2 antibody [6F8] (ab124362) at 1/1000 dilution

    Lane 1 : HepG2 cell extract
    Lane 2 : HeLa cell extract
    Lane 3 : HT29 cell extract
    Lane 4 : A549 cell extract
    Lane 5 : COS7 cell extract
    Lane 6 : Jurkat cell extract
    Lane 7 : MDCK cell extract
    Lane 8 : PC12 cell extract
    Lane 9 : MCF7 cell extract

    Lysates/proteins at 35 µg per lane.
    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC204703)
  • Staining of ERK2 in Paraffin-embedded Adenocarcinoma of Human breast tissue by Immunohistochemistry using ab124362 at a dilution of 1/50.
  • Staining of ERK2 in Paraffin-embedded Adenocarcinoma of Human ovary tissue by Immunohistochemistry using ab124362 at a dilution of 1/50.
  • Lane 1: controle IgG. Lane 2: Staining of ERK2 in 0.5ml of DDK tagged ERK2 overexpression lysates (at 1/5 dilution with HEK293T lysate) immunoprecipitated using 2 µg ab124362 and 0.1mg of goat anti-mouse conjugated magnetic beads. Predicted band size : 41 kDa.
  • HEK293T cells transfected with either ERK2 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by ab124362 at a dilution of 1/100, and then analyzed by flow cytometry.

Anti-ERK2 antibody [6F8] (ab124362)参考文献

ab124362 has not yet been referenced specifically in any publications.

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