Anti-ERK2抗体[0.T.86] (ab33062)

概述

  • 产品名称Anti-ERK2抗体[0.T.86]
    参阅全部 ERK2 一抗
  • 描述
    小鼠单克隆抗体[0.T.86] to ERK2
  • 特异性This antibody recognizes ERK2 encoded by the MAPK gene. The antibody only binds to denatured ERK2.
  • 经测试应用适用于: WB, IP, ELISAmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    HPLC purified recombinant full length protein (Mouse).

  • 阳性对照
    • WB: RIPA lysates from human A431 carcinoma cells. Also mouse 3T3 fibroblasts and rat L6 cells. IP: RIPA lysate from mouse 3T3 cells.

性能

应用

Our Abpromise guarantee covers the use of ab33062 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
IP
ELISA
  • 应用说明ELISA: Use at an assay dependent dilution.
    IP: Use 4 µg. Boil the cell lysate and let cool before adding the antibody
    WB: Use at a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 41 kDa. Add 2.5 µl of 2 mercaptoethanol per 100 µl of lysate and boil for 5 minutes to reduce the preparation. Load 20 µg of reduced lysate per lane for minigels.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1 (By similarity). Phosphorylates heat shock factor protein 4 (HSF4) and ARHGEF2.
      Acts as a transcriptional repressor. Binds to a [GC]AAA[GC] consensus sequence. Repress the expression of interferon gamma-induced genes. Seems to bind to the promoter of CCL5, DMP1, IFIH1, IFITM1, IRF7, IRF9, LAMP3, OAS1, OAS2, OAS3 and STAT1. Transcriptional activity is independent of kinase activity.
    • 序列相似性Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
      Contains 1 protein kinase domain.
    • 结构域The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
    • 翻译后修饰Dually phosphorylated on Thr-185 and Tyr-187, which activates the enzyme. Dephosphorylated by PTPRJ at Tyr-187.
    • 细胞定位Nucleus.
    • Information by UniProt
    • 数据库链接
    • 别名
      • ERK 2 antibody
      • ERK antibody
      • ERK-2 antibody
      • ERT1 antibody
      • Extracellular Signal Regulated Kinase 2 antibody
      • Extracellular signal-regulated kinase 2 antibody
      • MAP kinase 1 antibody
      • MAP kinase 2 antibody
      • MAP kinase isoform p42 antibody
      • MAPK 1 antibody
      • MAPK 2 antibody
      • Mapk1 antibody
      • MAPK2 antibody
      • Mitogen-activated protein kinase 1 antibody
      • Mitogen-activated protein kinase 2 antibody
      • MK01_HUMAN antibody
      • P38 antibody
      • P40 antibody
      • P41 antibody
      • p42-MAPK antibody
      • P42MAPK antibody
      • PRKM1 antibody
      • PRKM2 antibody
      • protein kinase, mitogen-activated, 1 antibody
      • protein kinase, mitogen-activated, 2 antibody
      • protein tyrosine kinase ERK2 antibody
      see all

    Anti-ERK2 antibody [0.T.86] (ab33062)参考文献

    ab33062 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"