Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187)抗体[G15-B] (ab136926)
Key features and details
- Rabbit monoclonal [G15-B] to Erk1 (pT202/pY204) + Erk2 (pT185/pY187)
- Suitable for: ICC/IF, WB
- Reacts with: Rat, Human
- Isotype: IgG
概述
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产品名称
Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187)抗体[G15-B]
参阅全部 Erk1 (pT202/pY204) + Erk2 (pT185/pY187) 一抗 -
描述
兔单克隆抗体[G15-B] to Erk1 (pT202/pY204) + Erk2 (pT185/pY187) -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, WBmore details -
种属反应性
与反应: Rat, Human
预测可用于: Mouse -
免疫原
Synthetic peptide corresponding to Human Erk1 (pT202/pY204) + Erk2 (pT185/pY187). Including the conserved pT-E-pY motif
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阳性对照
- PC12 cell lysates (treated with EGF, PMA or EGF); HEK293 cells treated with hydrogen peroxide.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 8.00
Preservative: 0.05% Sodium azide
Constituents: 0.32% Tris HCl, 1% BSA -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
This immunoglobulin is the product of one single B-cell line from the crude anti-peptide polyclonal anti-serum. This antibody is purified using a proprietary technique and offers a completely post-translationally modified and properly glycosylated antibody. This offers increased stability. -
克隆
单克隆 -
克隆编号
G15-B -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab136926于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ICC/IF |
1/100.
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WB |
1/5000. Predicted molecular weight: 41, 43 kDa.
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说明 |
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ICC/IF
1/100. |
WB
1/5000. Predicted molecular weight: 41, 43 kDa. |
图片
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All lanes : Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody [G15-B] (ab136926) at 1/5000 dilution
Lane 1 : PC12 cell lysate - untreated
Lane 2 : PC12 cell lysate - treated with EGF-100ng/ml (5min) in serum-free DMEM
Lane 3 : PC12 cell lysate - treated with PMA-100nM (30min) in serum-free DMEM
Lane 4 : PC12 cell lysate treated with EGF-100ng/ml (5min) with 10% FBS in DMEM
Lysates/proteins at 50 µg per lane.
Predicted band size: 41, 43 kDa -
Immunocytochemical analysis of HEK293 cells labelling phospho-ERK1 + ERK2 with ab136926 at 1/100 dilution.
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Immunocytochemical analysis of HEK293 cells (hydrogen peroxide treated) labelling phospho-ERK1 + ERK2 with ab136926 at 1/100 dilution.
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab136926 被引用在 4 文献中.
- Zhang Y et al. Transcription factor PAX4 facilitates gastric cancer progression through interacting with miR-27b-3p/Grb2 axis. Aging (Albany NY) 13:16786-16803 (2021). PubMed: 34162761
- Yu N et al. Serum amyloid A, an acute phase protein, stimulates proliferative and proinflammatory responses of keratinocytes. Cell Prolif 50:N/A (2017). PubMed: 27910163
- He X et al. HOXB7 overexpression promotes cell proliferation and correlates with poor prognosis in gastric cancer patients by inducing expression of both AKT and MARKs. Oncotarget 8:1247-1261 (2017). WB ; Human . PubMed: 27901487
- Venegas LA et al. A comparison of phosphospecific affinity reagents reveals the utility of recombinant Forkhead-associated domains in recognizing phosphothreonine-containing peptides. N Biotechnol 33:537-43 (2016). PubMed: 26772725