Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187)抗体(ab4819)

概述

  • 产品名称Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187)抗体
    参阅全部 Erk1 (pT202/pY204) + Erk2 (pT185/pY187) 一抗
  • 描述
    兔多克隆抗体to Erk1 (pT202/pY204) + Erk2 (pT185/pY187)
  • 经测试应用适用于: WB, IHC-Fr, Flow Cytmore details
  • 种属反应性
    与反应: Rat, Human, Culex pipiens
    预测可用于: a wide range of other species
  • 免疫原

    Synthetic peptide corresponding to Human Erk1 (pT202/pY204) + Erk2 (pT185/pY187). This region is conserved among many species including rat, mouse, cow, frog, snail, nematode, and fruit fly.
    (Peptide available as ab5313, ab5354, ab5255)

  • 阳性对照
    • PC12 cells +/- Sorbitol.

性能

应用

Our Abpromise guarantee covers the use of ab4819 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Predicted molecular weight: 44,42 kDa.
IHC-Fr Use at an assay dependent concentration.
Flow Cyt Use 10µl for 106 cells.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody 图像



  • Predicted band size : 44,42 kDa

    Peptide Competition: Extracts prepared from PC12 cells stimulated with NGF were resolved by SDS-PAGE on a 10% Tris-glycine gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer overnight at 4oC, then were incubated with 0.50 µg/mL ab4819 antibody for two hours at room temperature, following prior incubation with: no peptide (1), the non-phosphopeptide corresponding to the immunogen (2), a generic phosphothreonine containing peptide (3), a generic phosphotyrosine containing peptide (4), or, the phosphopeptide immunogen (5). After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG alkaline phosphatase and bands were detected using the Tropix WesternStar method. The data show that only the peptide corresponding to ab4819 blocks the antibody signal, thereby demonstrating the specificity of the antibody.

    Peptide Competition: Extracts prepared from PC12 cells stimulated with NGF were resolved by SDS-PAGE on
  • Figure shows immunohistochemical localization of active ab4819 in sections from rac1 expressing cardiomyopathic animals displaying the dilated phenotype. Green = p-ERK1/2, Red = nuclei, and Blue = alpha-actinin.

Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody (ab4819)参考文献

This product has been referenced in:
  • Haidari M  et al. Disruption of endothelial adherens junctions by high glucose is mediated by protein kinase C-ß-dependent vascular endothelial cadherin tyrosine phosphorylation. Cardiovasc Diabetol 13:112 (2014). Human . Read more (PubMed: 25027642) »
  • Báez A  et al. The CD27(+) memory B cells display changes in the gene expression pattern in elderly individuals. Immunology N/A:N/A (2014). Read more (PubMed: 25196729) »

See all 12 Publications for this product

Product Wall

Application Western blot
Loading amount 10000 cells
Gel Running Conditions Reduced Denaturing (12%)
Sample Mouse Cell lysate - whole cell (Mouse Neuroblastoma (Neuro2A))
Specification Mouse Neuroblastoma (Neuro2A)
Treatment Synthetic Spiro analogues
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 28°C
Username

Abcam user community

Verified customer

提交于 Sep 12 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (10%)
Sample Culex pipiens Tissue lysate - whole (whole fly homogenates)
Specification whole fly homogenates
Treatment 30 minutes after feeding with sheep blood
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Jiawen Xu

Verified customer

提交于 Aug 20 2013

Thank you for contacting us.

I am sorry to that the antibody seems not tobe working as expected.

As discussed over the phone, please send me some of your WB images and additional protocol information, so that I can pass this on to ...

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I am happy to help! Please feel free to contact me if you need anything further.

I am sorry this product did not contain the volume as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement.

To check the status of the order please contact our Customer S...

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DISCOUNT CODE: xxx
Expiration date: xxx

I am very pleased to hear you would like to accept our offer and test ab4819 in termites. This code will give you: 1 free PRIMARY ANTIBODY before the...

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Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Termite Tissue lysate - whole (termite)
Loading amount 40 µg
Specification termite
Gel Running Conditions Reduced Denaturing
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 6% · Temperature: 23°C
Username

Mr. Chris Mattison

Verified customer

提交于 Apr 09 2012

DISCOUNT CODE: *** Expiration date: March 30th, 2012 I am very pleased to hear you would like to accept our offer and test ab4819 in termite. This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submi...

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Application Western blot
Sample Human Cell lysate - whole cell (prostate)
Loading amount 25 µg
Specification prostate
Blocking step BSA as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 5%
Username

Abcam user community

Verified customer

提交于 Jan 03 2007

Thank you for contacting us with your query. There is only a small difference between these two antibodies if you are going to make a choice between the two. They will both recognise phosphorylated ERK1 and ERK2. ERK1 and ERK2 are phosphorylated within...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"