Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187)抗体[MAPK-YT] (ab50011)

概述

  • 产品名称
    Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187)抗体[MAPK-YT]
    参阅全部 Erk1 (pT202/pY204) + Erk2 (pT185/pY187) 一抗
  • 描述
    小鼠单克隆抗体[MAPK-YT] to Erk1 (pT202/pY204) + Erk2 (pT185/pY187)
  • 经测试应用
    适用于: ICC/IF, ELISA, WB, IP, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat, Cow, Human, Saccharomyces cerevisiae, Drosophila melanogaster
    预测可用于: Xenopus laevis, Zebrafish
  • 免疫原

    Synthetic peptide corresponding to Erk1 (pT202/pY204) + Erk2 (pT185/pY187) conjugated to Keyhole Limpet Haemocyanin (KLH). Synthetic peptide corresponds to the phosphorylated form of human ERK1 and ERK2 activation loop.
    Sequence:

    HTGFLpTEpYVAT

  • 表位
    The epitope recognized by ab50011 is located in the region of phosphorylated threonine and tyrosine residues within the regulatory site of active MAP kinase (Thr185 and Tyr187 in ERK-2).
  • 阳性对照
    • Rat brain extract, Drosophila wing imaginal disc.
  • 常规说明

    This product was changed from ascites to tissue culture supernatant on 25th October 2016. The following lot is from ascites and is still in stock as of 25th October 2016- GR185138. Lot numbers higher than GR185138 will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.

性能

应用

Our Abpromise guarantee covers the use of ab50011 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/200.
ELISA Use at an assay dependent concentration.
WB 1/10000. Predicted molecular weight: 44 kDa.
IP Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.

Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody [MAPK-YT] 图像

  • Lane 1 : Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody [MAPK-YT] (ab50011) at 1/10000 dilution
    Lane 2 : no primary antibody

    Lane 1 : Extract of Rat1 cells activated with 100µM Vanadate+200µM H2O2
    Lane 2 : Extract of Rat1 cells activated with 100µM Vanadate+200µM H2O2

    Secondary
    Goat Anti-Mouse IgG, AlkPhos Conjugate

    Predicted band size : 44 kDa
    Observed band size : 42,44 kDa (why is the actual band size different from the predicted?)
  • In situ immunostaining of the activated form of MAP kinase with ab50011 (green) in Drosophila wing imaginal disc (500 micrometers long).
  • ICC/IF of NRK cells labeling the activated form of MAP kinase with ab50011 (1/1000 dilution). The antibody was developed using Goat Anti-Mouse IgG, Cy3™ conjugate. Cells were counterstained with DAPI (blue).

Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody [MAPK-YT] (ab50011)参考文献

This product has been referenced in:
  • Ding Y  et al. Effects of 1,25(OH)2 D3 and vitamin D receptor on peripheral CD4(+) /CD8(+) double-positive T lymphocytes in a mouse model of systemic lupus erythematosus. J Cell Mol Med 21:975-985 (2017). WB ; Mouse . Read more (PubMed: 28063200) »
  • Antony J  et al. The GAS6-AXL signaling network is a mesenchymal (Mes) molecular subtype-specific therapeutic target for ovarian cancer. Sci Signal 9:ra97 (2016). WB . Read more (PubMed: 27703030) »

See all 25 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (skin)
Permeabilization
No
Specification
skin
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Aceton methanol
Username

Abcam user community

Verified customer

提交于 Feb 06 2017

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Dako REALPeroxidase-Blocking Solution S2023 as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 26°C
Antigen retrieval step
Heat mediated
Sample
Zebrafish Tissue sections (Liver, dysplatic cells)
Specification
Liver, dysplatic cells
Permeabilization
No
Fixative
Paraformaldehyde
Username

Dr. Inhye Jung

Verified customer

提交于 Jul 17 2014

Thank you for your reply and further question.

This monoclonal antibody in the ascites fluid that was produced by the hybridoma within the mouse peritoneal cavity. As far as I can tell there should only be the specific antibody present in te...

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Thank you for your enquiry.

I can confirm that the total protein concentration in ab50011 should be between 15 - 55 mg/ml when measured by Biuret and that the IgG1 concentration should be between 3 - 6 mg/ml.

Regrettably we do not...

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Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this...

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Thank you for your reply.

I am very sorry to confirm that we sell a lot of this antibody and regrettably we now have no vials of GR121-7 left.

As previously discussed, I can confirm that our lots GR121-7 and GR121-8 are the same o...

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Thank you for taking the time to complete our questionnaire and provide the images.

The details you have kindly provided will provide us with vital information for our monitoring of product quality.

I appreciate the time you have ...

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Thank you for taking the time to contact us. You enquiry has been forwarded to the scientific support team. I am sorry to hear you have had difficulty obtaining satisfactory results from this vial of antibody.

I appreciate the time you have ...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Tissue lysate - whole (Liver)
Loading amount
50 µg
Specification
Liver
Gel Running Conditions
Reduced Denaturing (12)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 Jun 25 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rat Tissue lysate - whole (Liver)
Loading amount
50 µg
Specification
Liver
Gel Running Conditions
Reduced Denaturing (12)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 Jun 25 2012

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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