Anti-ERK1 (phospho Y204) + ERK2 (phospho Y187)抗体(ab47339)

概述

  • 产品名称
    Anti-ERK1 (phospho Y204) + ERK2 (phospho Y187)抗体
    参阅全部 ERK1+ERK2 一抗
  • 描述
    兔多克隆抗体to ERK1 (phospho Y204) + ERK2 (phospho Y187)
  • 特异性
    This antibody is specific for ERK1, only when phosphorylated at tyrosine 204
  • 经测试应用
    适用于: IHC-P, WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    A synthesized phosphopeptide derived around the phosphorylation site of ERK1 tyrosine 204 and ERK2 tyrosine 187 (T-E-YP-V-A).

  • 阳性对照
    • Extracts from Jurkat cells

性能

应用

Our Abpromise guarantee covers the use of ab47339 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use at an assay dependent concentration.
WB 1/500 - 1/1000. Detects a band of approximately 44, 42 kDa (predicted molecular weight: 44, 42 kDa).
ELISA 1/10000.

靶标

图片

  • All lanes : Anti-ERK1 (phospho Y204) + ERK2 (phospho Y187) antibody (ab47339) at 1/500 dilution

    Lane 1 : Extracts from Jurkat cells treated with PMA (200ng/ml, 15min)
    Lane 2 : Extracts from Jurkat cells un-treated


    Predicted band size : 44, 42 kDa
    Observed band size : 42,44 kDa
  • Ab47339 staining human normal or colon tissue. Staining is localised to cytoplasm.
    Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control.
    Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
  • All lanes : Anti-ERK1 (phospho Y204) + ERK2 (phospho Y187) antibody (ab47339)

    Lane 1 : Jurkat cell extract
    Lane 2 : Jurkat cell extract treated with PMA


    Predicted band size : 44, 42 kDa
    Observed band size : 43 kDa (why is the actual band size different from the predicted?)

文献

This product has been referenced in:
  • Ferraiuolo RM  et al. The cyclin-like protein, SPY1, regulates the ERa and ERK1/2 pathways promoting tamoxifen resistance. Oncotarget 8:23337-23352 (2017). WB ; Human . Read more (PubMed: 28423577) »
  • Li R  et al. Secreted GRP78 activates EGFR-SRC-STAT3 signaling and confers the resistance to sorafeinib in HCC cells. Oncotarget 8:19354-19364 (2017). WB ; Human . Read more (PubMed: 28423613) »

See all 6 Publications for this product

客户评价及客户问答

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 26°C
Sample
Human Cell (Human Granulosa Cell Line)
Specification
Human Granulosa Cell Line
Permeabilization
Yes - Ethanol and Triton
Fixative
Paraformaldehyde
Username

Dr. Francesco Elia Marino

Verified customer

提交于 Jun 10 2014

Application
Western blot
Loading amount
60 µg
Gel Running Conditions
Reduced Non-Denaturing (Native) (12)
Sample
Human Cell lysate - whole cell (Human granulosa cell line)
Specification
Human granulosa cell line
Blocking step
Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 26°C
Username

Dr. Francesco Elia Marino

Verified customer

提交于 Jun 02 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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