The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration. Predicted molecular weight: 42 , 44 kDa.
Use at an assay dependent dilution.
功能Involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1 (By similarity). Phosphorylates heat shock factor protein 4 (HSF4) and ARHGEF2. Acts as a transcriptional repressor. Binds to a [GC]AAA[GC] consensus sequence. Repress the expression of interferon gamma-induced genes. Seems to bind to the promoter of CCL5, DMP1, IFIH1, IFITM1, IRF7, IRF9, LAMP3, OAS1, OAS2, OAS3 and STAT1. Transcriptional activity is independent of kinase activity.
序列相似性Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily. Contains 1 protein kinase domain.
结构域The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
翻译后修饰Dually phosphorylated on Thr-185 and Tyr-187, which activates the enzyme. Dephosphorylated by PTPRJ at Tyr-187.
Western blot - Anti-ERK1 + ERK2 antibody [SB46b] (Biotin) (ab82524)
Predicted band size : 42 , 44 kDa
Total cell lysates from Jurkat cells were resolved by electrophoresis, transferred to PVDF membrane, and probed with Anti-ERK1 + ERK2 antibody [SB46b] (Biotin) (ab82524). Proteins were visualized using Mouse anti-biotin-HRP secondary antibody and chemiluminescent detection.