Anti-ERK1 + ERK2抗体[ERK-7D8] (ab54230)

概述

  • 产品名称Anti-ERK1 + ERK2抗体[ERK-7D8]
    参阅全部 ERK1 + ERK2 一抗
  • 描述
    小鼠单克隆抗体[ERK-7D8] to ERK1 + ERK2
  • 特异性ab54230 reacts strongly with both ERK1 and ERK2.
  • 经测试应用适用于: WB, IP, ELISA, EMSA, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Dog, Human, Zebrafish
    预测可用于: Cow, Carp, Chimpanzee, Sea Urchin, Opossum
  • 免疫原

    Synthetic peptide:

    EALAHPYLEQYYDPTDEPVAEE

    , corresponding to internal sequence amino acids 324-345, a segment near the C-terminus of Rat ERK1

  • 阳性对照
    • Human A431 cell lysates, Human K562 cell lysates, Canine MDCK cells lysates, Mouse brain lysate, Rat brain lysate.

性能

应用

Our Abpromise guarantee covers the use of ab54230 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 44 kDa.
IP Use at 2-5 µg/mg of lysate.
ELISA Use a concentration of 0.1 - 1 µg/ml.
EMSA Use at an assay dependent dilution.
ICC/IF Use at an assay dependent concentration.

靶标

  • 功能Involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1 (By similarity). Phosphorylates heat shock factor protein 4 (HSF4) and ARHGEF2.
    Acts as a transcriptional repressor. Binds to a [GC]AAA[GC] consensus sequence. Repress the expression of interferon gamma-induced genes. Seems to bind to the promoter of CCL5, DMP1, IFIH1, IFITM1, IRF7, IRF9, LAMP3, OAS1, OAS2, OAS3 and STAT1. Transcriptional activity is independent of kinase activity.
  • 序列相似性Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
    Contains 1 protein kinase domain.
  • 结构域The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
  • 翻译后修饰Dually phosphorylated on Thr-185 and Tyr-187, which activates the enzyme. Dephosphorylated by PTPRJ at Tyr-187.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ERK 1 antibody
    • ERK 2 antibody
    • ERK-2 antibody
    • ERK1 antibody
    • erk1/2 antibody
    • ERK2 antibody
    • ERT1 antibody
    • ERT2 antibody
    • Extracellular signal regulated kinase 1 antibody
    • Extracellular signal-regulated kinase 2 antibody
    • MAP kinase 1 antibody
    • MAP kinase 2 antibody
    • MAP kinase isoform p42 antibody
    • MAP kinase isoform p44 antibody
    • MAPK 1 antibody
    • MAPK 2 antibody
    • MAPK 3 antibody
    • Mapk1 antibody
    • MAPK2 antibody
    • MAPK3 antibody
    • Mitogen-activated protein kinase 1 antibody
    • Mitogen-activated protein kinase 2 antibody
    • MK01_HUMAN antibody
    • p38 antibody
    • p40 antibody
    • p41 antibody
    • p42-MAPK antibody
    • PRKM 2 antibody
    see all

Anti-ERK1 + ERK2 antibody [ERK-7D8] 图像

  • All lanes : Anti-ERK1 + ERK2 antibody [ERK-7D8] (ab54230) at 1/1000 dilution

    Lane 1 : Human granulosa cell line whole cell lysate
    Lane 2 : Human granulosa cell line whole cell lysate

    Lysates/proteins at 60 µg per lane.

    Secondary
    IRDye® 680-conjugated goat anti-mouse IgG1 (H+L) polyclonal at 1/25000 dilution

    Performed under reducing conditions.

    Predicted band size : 44 kDa
    Observed band size : 44 kDa


    Exposure time : 4 minutes

    This image is courtesy of an Abreview submitted by Francesco Elia Marino

    See Abreview

Anti-ERK1 + ERK2 antibody [ERK-7D8] (ab54230)参考文献

This product has been referenced in:
  • Liu L  et al. Spring viraemia of carp virus induces autophagy for necessary viral replication. Cell Microbiol 17:595-605 (2015). Read more (PubMed: 25376386) »
  • Chamberlain CE  et al. Menin determines K-RAS proliferative outputs in endocrine cells. J Clin Invest 124:4093-101 (2014). WB ; Mouse . Read more (PubMed: 25133424) »

See all 6 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Blocking step Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 26°C
Sample Human Cell (Human granulosa cell Line)
Specification Human granulosa cell Line
Permeabilization Yes - Ethanol and Triton
Fixative Paraformaldehyde
Username

Dr. FRANCESCO ELIA MARINO

Verified customer

提交于 Jun 19 2014

Application Western blot
Loading amount 60 µg
Gel Running Conditions Reduced Non-Denaturing (Native) (12)
Sample Human Cell lysate - whole cell (Human Granulosa cell line)
Specification Human Granulosa cell line
Blocking step Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 26°C
Username

Dr. FRANCESCO ELIA MARINO

Verified customer

提交于 Jun 02 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"