Anti-ERK1 + ERK2抗体(ab115799)

概述

  • 产品名称Anti-ERK1 + ERK2抗体
    参阅全部 ERK1 + ERK2 一抗
  • 描述
    兔多克隆抗体to ERK1 + ERK2
  • 经测试应用适用于: WB, IP, IHC-P, Flow Cytmore details
  • 种属反应性
    与反应: Mouse, Rat, Cow, Human
    预测可用于: Sheep, Chicken, Hamster, Xenopus laevis, Drosophila melanogaster
  • 免疫原

    Synthetic peptide corresponding to a region of Rat ERK1 + ERK2.

  • 阳性对照
    • Human A431 fibroblast cell lysate; Human breast cancer tissue; U251 cells.

性能

应用

Our Abpromise guarantee covers the use of ab115799 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Detects a band of approximately 42, 44 kDa (predicted molecular weight: 41 kDa).
IP Use at an assay dependent concentration.
IHC-P 1/50.
Flow Cyt Use a concentration of 10 µg/ml. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

靶标

  • 功能Involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1 (By similarity). Phosphorylates heat shock factor protein 4 (HSF4) and ARHGEF2.
    Acts as a transcriptional repressor. Binds to a [GC]AAA[GC] consensus sequence. Repress the expression of interferon gamma-induced genes. Seems to bind to the promoter of CCL5, DMP1, IFIH1, IFITM1, IRF7, IRF9, LAMP3, OAS1, OAS2, OAS3 and STAT1. Transcriptional activity is independent of kinase activity.
  • 序列相似性Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
    Contains 1 protein kinase domain.
  • 结构域The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
  • 翻译后修饰Dually phosphorylated on Thr-185 and Tyr-187, which activates the enzyme. Dephosphorylated by PTPRJ at Tyr-187.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ERK 1 antibody
    • ERK 2 antibody
    • ERK-2 antibody
    • ERK1 antibody
    • erk1/2 antibody
    • ERK2 antibody
    • ERT1 antibody
    • ERT2 antibody
    • Extracellular signal regulated kinase 1 antibody
    • Extracellular signal-regulated kinase 2 antibody
    • MAP kinase 1 antibody
    • MAP kinase 2 antibody
    • MAP kinase isoform p42 antibody
    • MAP kinase isoform p44 antibody
    • MAPK 1 antibody
    • MAPK 2 antibody
    • MAPK 3 antibody
    • Mapk1 antibody
    • MAPK2 antibody
    • MAPK3 antibody
    • Mitogen-activated protein kinase 1 antibody
    • Mitogen-activated protein kinase 2 antibody
    • MK01_HUMAN antibody
    • p38 antibody
    • p40 antibody
    • p41 antibody
    • p42-MAPK antibody
    • PRKM 2 antibody
    see all

Anti-ERK1 + ERK2 antibody 图像

  • Anti-ERK1 + ERK2 antibody (ab115799) at 1/1000 dilution + Human A431 fibroblast cell lysate

    Predicted band size : 41 kDa
  • ab115799 at 1/50 dilution staining ERK1 + ERK2 in Paraffin-embedded Human breast cancer tissue by Immunohistochemistry.
  • Flow cytometry analysis of U251 cells using ab115799 at 10ug/ml (right) and control rabbit IgG (left).

Anti-ERK1 + ERK2 antibody (ab115799)参考文献

ab115799 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab115799.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"