Anti-ERK1抗体[12D11] - ChIP Grade (ab119357)

概述

  • 产品名称Anti-ERK1抗体[12D11] - ChIP Grade
    参阅全部 ERK1 一抗
  • 描述
    小鼠单克隆抗体[12D11] to ERK1 - ChIP Grade
  • 经测试应用适用于: WB, ICC/IF, IHC-P, ChIPmore details
  • 种属反应性
    与反应: Mouse, Human, Non Human Primates
  • 免疫原

    Recombinant full length Human ERK1 expressed in bacteria.

  • 阳性对照
    • 293, U2OS, K562, A549, HeLa, Jurkat, MCF7, HepG2, MEF, NIH 3T3, C2C12 and COS7 cell lysates; HeLa cells; Human colon cancer tissue.

性能

应用

Our Abpromise guarantee covers the use of ab119357 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Predicted molecular weight: 43 kDa.
ICC/IF 1/100.
IHC-P 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ChIP Use at an assay dependent concentration.

1-3 μl

靶标

  • 功能Involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1 (By similarity). Phosphorylates heat shock factor protein 4 (HSF4).
  • 序列相似性Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
    Contains 1 protein kinase domain.
  • 结构域The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
  • 翻译后修饰Dually phosphorylated on Thr-202 and Tyr-204, which activates the enzyme. Dephosphorylated by PTPRJ at Tyr-204.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ERK 1 antibody
    • ERK antibody
    • ERK-1 antibody
    • ERK1 antibody
    • ERT 2 antibody
    • ERT2 antibody
    • Extracellular Signal Regulated Kinase 1 antibody
    • Extracellular signal related kinase 1 antibody
    • Extracellular signal-regulated kinase 1 antibody
    • HGNC6877 antibody
    • HS44KDAP antibody
    • HUMKER1A antibody
    • Insulin Stimulated MAP2 Kinase antibody
    • Insulin-stimulated MAP2 kinase antibody
    • MAP kinase 1 antibody
    • MAP kinase 3 antibody
    • MAP Kinase antibody
    • MAP kinase isoform p44 antibody
    • MAPK 1 antibody
    • MAPK 3 antibody
    • MAPK antibody
    • MAPK1 antibody
    • Mapk3 antibody
    • MGC20180 antibody
    • Microtubule Associated Protein 2 Kinase antibody
    • Microtubule-associated protein 2 kinase antibody
    • Mitogen Activated Protein Kinase 3 antibody
    • Mitogen-activated protein kinase 1 antibody
    • Mitogen-activated protein kinase 3 antibody
    • MK03_HUMAN antibody
    • OTTHUMP00000174538 antibody
    • OTTHUMP00000174541 antibody
    • p44 ERK1 antibody
    • p44 MAPK antibody
    • p44-ERK1 antibody
    • p44-MAPK antibody
    • P44ERK1 antibody
    • P44MAPK antibody
    • PRKM 3 antibody
    • PRKM3 antibody
    • Protein Kinase Mitogen Activated 3 antibody
    see all

Anti-ERK1 antibody [12D11] - ChIP Grade 图像

  • All lanes : Anti-ERK1 antibody [12D11] - ChIP Grade (ab119357) at 1/1000 dilution

    Lane 1 : 293T cell lysate
    Lane 2 : U2OS cell lysate
    Lane 3 : K562 cell lysate
    Lane 4 : A549 cell lysate
    Lane 5 : HeLa cell lysate
    Lane 6 : Jurkat cell lysate
    Lane 7 : MCF7 cell lysate
    Lane 8 : HepG2 cell lysate
    Lane 9 : MEF cell lysate
    Lane 10 : NIH 3T3 cell lysate
    Lane 11 : C2C12 cell lysate
    Lane 12 : COS7 cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat anti-mouse-HRP at 1/25000 dilution
    Developed using the ECL technique

    Predicted band size : 43 kDa
  • ab119357, at 1/100 dilution, staining ERK1 in untreated and siRNA knockdown treated HeLa cells (Formalin fixed and permeabilized with 0.1% Triton X-100) by Immunoflourescence followed by DyLight 549 goat-anti-mouse secondary antibody at a dilution of 1/400.
    Nuclei (blue) were stained with Hoechst 33342 dye.
  • ab119357, at 1/100 dilution, staining ERK1 in deparaffinized Human colon cancer tissue by Immunohistochemistry using a goat anti-mouse HRP secondary antibody at a dilution of 1/500 and colorimetric detection using metal enhanced DAB.
    Note: strong cytoplasmic localization of ERK1.

Anti-ERK1 antibody [12D11] - ChIP Grade (ab119357)参考文献

ab119357 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab119357.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"