概述

  • 产品名称
    Anti-eRF3/GSPT1抗体
    参阅全部 eRF3/GSPT1 一抗
  • 描述
    兔多克隆抗体to eRF3/GSPT1
  • 经测试应用
    适用于: WB, IP, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide corresponding to Human eRF3/GSPT1 aa 190-204 (internal sequence) conjugated to keyhole limpet haemocyanin (Cysteine residue).
    Sequence:

    FETGFEKGGQTREHA


    Database link: P15170

  • 阳性对照
    • WB: HeLa whole cell lysate.

性能

应用

Our Abpromise guarantee covers the use of ab49878 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 55 kDa.
IP Use a concentration of 1 - 2 µg/ml.
ICC/IF Use a concentration of 5 - 10 µg/ml. Paraformadehyde fixed cells

靶标

  • 相关性
    Translation termination in eukaryotes is governed by two proteins, belonging to the class-1 (eRF1) and class-2 (eRF3) polypeptide release factors. eRF3/GSPT1 is involved in regulation of mammalian cell growth and belongs to the GTP-binding elongation factor family (ERF3 subfamily). eRF3 catalyzes hydrolysis of GTP to GDP and inorganic phosphate in the ribosome in the absence of mRNA, tRNA, aminoacyl-tRNA and peptidyl-tRNA.
  • 细胞定位
    Cytoplasmic
  • 数据库链接
  • 别名
    • 551G9.2 antibody
    • Anti Eukaryotic Release Factor 3a antibody
    • Anti G1 to S phase transition 1 antibody
    • Anti GST1, homolog of yeast antibody
    • eRF 3a antibody
    • eRF3a antibody
    • G1 to S phase transition 1 antibody
    • G1 to S phase transition protein 1 homolog antibody
    • G1 to S phase transition protein antibody
    • GSPT 1 antibody
    • GSPT1 antibody
    • GST 1 antibody
    • GST1 antibody
    • GTP binding protein GST1 HS antibody
    • Transition protein 1 antibody
    see all

文献

This product has been referenced in:
  • Nguyen TV  et al. p97/VCP promotes degradation of CRBN substrate glutamine synthetase and neosubstrates. Proc Natl Acad Sci U S A 114:3565-3571 (2017). WB ; Human . Read more (PubMed: 28320958) »
  • Geißler V  et al. The RNA helicase Ddx5/p68 binds to hUpf3 and enhances NMD of Ddx17/p72 and Smg5 mRNA. Nucleic Acids Res 41:7875-88 (2013). WB ; Human . Read more (PubMed: 23788676) »

See all 5 Publications for this product

客户评价及客户问答

Application
Western blot
Sample
Human Cell lysate - whole cell (Lymphoblast)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
15 µg
Specification
Lymphoblast
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Jan 20 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa cells)
Loading amount
1.7e+006 cells
Specification
HeLa cells
Gel Running Conditions
Reduced Denaturing (10% SDS-PAGE)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Ms. Luisa Romao

Verified customer

提交于 Mar 07 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册