Anti-ERF (phospho T526)抗体(ab59178)

概述

  • 产品名称Anti-ERF (phospho T526)抗体
    参阅全部 ERF 一抗
  • 描述
    兔多克隆抗体to ERF (phospho T526)
  • 特异性Detects endogenous levels of ERF only when phosphorylated at threonine 526.
  • 经测试应用适用于: IHC-P, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse
  • 免疫原

    Synthetic phosphopeptide from around the phosphorylation site of threonine 526 (PLTPPR) of human ERF

  • 阳性对照
    • Human lung carcinoma tissue

性能

应用

Our Abpromise guarantee covers the use of ab59178 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use at an assay dependent concentration.
ELISA 1/20000.

靶标

  • 功能Potent transcriptional repressor that binds to the H1 element of the Ets2 promoter. May regulate other genes involved in cellular proliferation. Required for extraembryonic ectoderm differentiation, ectoplacental cone cavity closure, and chorioallantoic attachment (By similarity). May be important for regulating trophoblast stem cell differentiation.
  • 组织特异性Highest levels in testis, ovary, pancreas, and heart.
  • 序列相似性Belongs to the ETS family.
    Contains 1 ETS DNA-binding domain.
  • 翻译后修饰Phosphorylated by multiple kinases including MAPK1/ERK2 at THR-526. Phosphorylation regulates the activity of ERF.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ERF antibody
    • ERF_HUMAN antibody
    • ETS domain containing transcription factor ERF antibody
    • ETS domain-containing transcription factor ERF antibody
    • Ets2 repressor factor antibody
    • PE 2 antibody
    • PE-2 antibody
    • PE2 antibody
    see all

Anti-ERF (phospho T526) antibody 图像

  • Immunohistochemical analysis of paraffin embedded human lung carcinoma tissue using ab59178 at 1/50 dilution. Left: untreated. Right: sample treated with immunising peptide.

Anti-ERF (phospho T526) antibody (ab59178)参考文献

ab59178 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Human Cell lysate - whole cell (HEK293 cells)
Loading amount 35 µg
Specification HEK293 cells
Treatment 100ng/ml nocodazole 6 hours
Gel Running Conditions Reduced Denaturing (4-12% Bis-Tris)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
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提交于 May 06 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"