概述

  • 产品名称
  • 描述
    小鼠多克隆抗体to ERCC6L
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Full length Human ERCC6L protein (AAH08808.1, amino acids 1-419)

  • 阳性对照
    • Hela cell lysate, ERCC6L transfected 293T cell lysate.

性能

应用

Our Abpromise guarantee covers the use of ab88560 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 141 kDa.

靶标

  • 功能
    DNA helicase that acts as an essential component of the spindle assembly checkpoint. Contributes to the mitotic checkpoint by recruiting MAD2 to kinetochores and monitoring tension on centromeric chromatin. Acts as a tension sensor that associates with catenated DNA which is stretched under tension until it is resolved during anaphase.
  • 序列相似性
    Belongs to the SNF2/RAD54 helicase family.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
    Contains 2 TPR repeats.
  • 翻译后修饰
    Phosphorylation by PLK1 prevents the association with chromosome arms and restricts its localization to the kinetochore-centromere region.
  • 细胞定位
    Chromosome > centromere. Chromosome > centromere > kinetochore. Localizes to kinetochores, inner centromeres and thin threads connecting separating chromosomes even during anaphase. In prometaphase cells, it mostly concentrates in between kinetochores. In metaphase, it localizes to numerous thin threads that stretch between sister kinetochores of the aligned chromosomes and are composed of catenated centromeric DNA. Evolution from inner centromeres to thin threads takes place in response to tension. Resolution of thin threads requires topoisomerase 2-alpha (TOP2A) after anaphase onset.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ATP dependent helicase ERCC6 like antibody
    • ATP-dependent helicase ERCC6-like antibody
    • DNA excision repair protein ERCC 6 like antibody
    • DNA excision repair protein ERCC-6-like antibody
    • ERC6L_HUMAN antibody
    • ERCC 6L antibody
    • Ercc6l antibody
    • Excision repair cross complementing rodent repair deficiency complementation group 6 like antibody
    • Excision repair protein ERCC6 like antibody
    • FLJ20105 antibody
    • MGC131695 antibody
    • PICH antibody
    • Plk1 interacting checkpoint helicase antibody
    • PLK1-interacting checkpoint helicase antibody
    • SNF2/RAD54 family protein antibody
    • Tumor antigen BJ HCC 15 antibody
    • Tumor antigen BJ-HCC-15 antibody
    see all

图片

  • All lanes : Anti-ERCC6L antibody (ab88560) at 1 µg/ml

    Lane 1 : ERCC6L transfected 293T cell lysate
    Lane 2 : Non transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 141 kDa
    Observed band size : 60 kDa (why is the actual band size different from the predicted?)
  • Anti-ERCC6L antibody (ab88560) at 1 µg/ml + Hela cell lysate at 50 µg

    Predicted band size : 141 kDa
    Observed band size : 141 kDa
    Additional bands at : 57 kDa. We are unsure as to the identity of these extra bands.

实验方案

文献

ab88560 has not yet been referenced specifically in any publications.

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