概述

  • 产品名称Anti-ERCC1抗体
    参阅全部 ERCC1 一抗
  • 描述
    兔多克隆抗体to ERCC1
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Hamster, Cow
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 250 to the C-terminus of Human ERCC1.

    (Peptide available as ab93790.)

  • 阳性对照
    • This antibody gave a positive signal mouse kidney tissue lysate and in the following whole cell lysates: HeLa; Jurkat.

性能

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应用

Our Abpromise guarantee covers the use of ab69398 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 32 kDa (predicted molecular weight: 32 kDa). Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

靶标

  • 功能Structure-specific DNA repair endonuclease responsible for the 5'-incision during DNA repair.
  • 疾病相关Defects in ERCC1 are the cause of cerebro-oculo-facio-skeletal syndrome type 4 (COFS4) [MIM:610758]. COFS is a degenerative autosomal recessive disorder of prenatal onset affecting the brain, eye and spinal cord. After birth, it leads to brain atrophy, hypoplasia of the corpus callosum, hypotonia, cataracts, microcornea, optic atrophy, progressive joint contractures and growth failure. Facial dysmorphism is a constant feature. Abnormalities of the skull, eyes, limbs, heart and kidney also occur.
  • 序列相似性Belongs to the ERCC1/RAD10/SWI10 family.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • COFS 4 antibody
    • COFS4 antibody
    • DNA excision repair protein ERCC 1 antibody
    • DNA excision repair protein ERCC-1 antibody
    • DNA excision repair protein ERCC1 antibody
    • ERCC 1 antibody
    • ERCC1 antibody
    • ERCC1_HUMAN antibody
    • Excision repair cross complementation group 1 antibody
    • Excision repair cross complementing 1 antibody
    • Excision Repair Cross Complementing Rodent Repair Deficiency Complementation Group 1 antibody
    • Excision repair protein antibody
    • RAD 10 antibody
    • RAD10 antibody
    • UV 20 antibody
    • UV20 antibody
    see all

Anti-ERCC1 antibody 图像

  • All lanes : Anti-ERCC1 antibody (ab69398) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 32 kDa
    Observed band size : 32 kDa
    Additional bands at : 36 kDa,55 kDa,90 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 10 minutes
  • Anti-ERCC1 antibody (ab69398) at 1 µg/ml + Kidney (Mouse) Tissue Lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 32 kDa
    Observed band size : 36 kDa (why is the actual band size different from the predicted?)


    Exposure time : 20 minutes

Anti-ERCC1 antibody (ab69398)参考文献

ab69398 has not yet been referenced specifically in any publications.

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