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Our Abpromise guarantee covers the use of ab2356 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 µg/ml.|
|WB||1/100 - 1/500. Predicted molecular weight: 33-36 kDa.|
|IP||1/100 - 1/500.|
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|IHC-Fr||1/50 - 1/100. 1/50 - 1/100 in an ABC method. We suggest an incubation period of 30 minutes at room temperature.|
|Flow Cyt||Use 1µg for 106 cells.
ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human tonsil tissue with ab2356 labelling ERCC1.
Immunohistochemical analysis of formalin fixed paraffin embedded human tonsil tissue sections with ab2356 labelling ERCC1.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"