Anti-ERCC1抗体[4F9] (ab113941)

概述

  • 产品名称Anti-ERCC1抗体[4F9]
    参阅全部 ERCC1 一抗
  • 描述
    小鼠单克隆抗体[4F9] to ERCC1
  • 经测试应用适用于: WB, IHC-P, ICC/IF, Flow Cytmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant full length Human ERCC1 protein expressed in 293T cells (NM_001983).

  • 阳性对照
    • HeLa, HT29, Jurkat and MCF7 cell lysates; HEK293T cell lysate transfected with pCMV6-ENTRY ERCC1; Human colon adenocarcinoma, kidney, lung, lung carcinoma, ovary, ovarian adenocarcinoma, pancreas, thyroid, endometrium adenocarcinoma, prostate, bladder and bladder carcinoma tissues; HeLa cells and COS7 cells transiently transfected by pCMV6-ENTRY ERCC1.

性能

应用

Our Abpromise guarantee covers the use of ab113941 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000. Predicted molecular weight: 33 kDa.
IHC-P 1/50.
ICC/IF 1/100.
Flow Cyt Use 0.1µg for 106 cells.

靶标

  • 功能Structure-specific DNA repair endonuclease responsible for the 5'-incision during DNA repair.
  • 疾病相关Defects in ERCC1 are the cause of cerebro-oculo-facio-skeletal syndrome type 4 (COFS4) [MIM:610758]. COFS is a degenerative autosomal recessive disorder of prenatal onset affecting the brain, eye and spinal cord. After birth, it leads to brain atrophy, hypoplasia of the corpus callosum, hypotonia, cataracts, microcornea, optic atrophy, progressive joint contractures and growth failure. Facial dysmorphism is a constant feature. Abnormalities of the skull, eyes, limbs, heart and kidney also occur.
  • 序列相似性Belongs to the ERCC1/RAD10/SWI10 family.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • COFS 4 antibody
    • COFS4 antibody
    • DNA excision repair protein ERCC 1 antibody
    • DNA excision repair protein ERCC-1 antibody
    • DNA excision repair protein ERCC1 antibody
    • ERCC 1 antibody
    • ERCC1 antibody
    • ERCC1_HUMAN antibody
    • Excision repair cross complementation group 1 antibody
    • Excision repair cross complementing 1 antibody
    • Excision Repair Cross Complementing Rodent Repair Deficiency Complementation Group 1 antibody
    • Excision repair protein antibody
    • RAD 10 antibody
    • RAD10 antibody
    • UV 20 antibody
    • UV20 antibody
    see all

Anti-ERCC1 antibody [4F9] 图像

  • Overlay histogram showing HeLa cells stained with ab113941 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab113941, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
  • All lanes : Anti-ERCC1 antibody [4F9] (ab113941) at 1/2000 dilution

    Lane 1 : HEK293T cell lysate, transfected with pCMV6-ENTRY control
    Lane 2 : HEK293T cell lysate, transfected with pCMV6-ENTRY ERCC1

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 33 kDa
    HEK293T cells were transfected with the relevant cDNA for 48 hrs and lysed.
  • All lanes : Anti-ERCC1 antibody [4F9] (ab113941) at 1/2000 dilution

    Lane 1 : HepG2 cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : HT29 cell lysate
    Lane 4 : A549 cell lysate
    Lane 5 : COS7 cell lysate
    Lane 6 : Jurkat cell lysate
    Lane 7 : MDCK cell lysate
    Lane 8 : PC12 cell lysate
    Lane 9 : MCF7 cell lysate

    Lysates/proteins at 35 µg per lane.


    Predicted band size : 33 kDa
  • ab113941, at 1/50, staining ERCC1 in Paraffin-embedded Human colon adenocarcinoma tissue by Immunohistochemistry.
  • ab113941, at 1/50, staining ERCC1 in Paraffin-embedded Human kidney tissue by Immunohistochemistry.
  • ab113941, at 1/50, staining ERCC1 in Paraffin-embedded Human lung tissue by Immunohistochemistry.
  • ab113941, at 1/50, staining ERCC1 in Paraffin-embedded Human lung carcinoma tissue by Immunohistochemistry.
  • ab113941, at 1/50, staining ERCC1 in Paraffin-embedded Human ovary tissue by Immunohistochemistry.
  • ab113941, at 1/50, staining ERCC1 in Paraffin-embedded Human ovary adenocarcinoma tissue by Immunohistochemistry.
  • ab113941, at 1/50, staining ERCC1 in Paraffin-embedded Human pancreas tissue by Immunohistochemistry.
  • ab113941, at 1/50, staining ERCC1 in Paraffin-embedded Human thyroid tissue by Immunohistochemistry.
  • ab113941, at 1/50, staining ERCC1 in Paraffin-embedded Human endometrium adenocarcinoma tissue by Immunohistochemistry.
  • ab113941, at 1/50, staining ERCC1 in Paraffin-embedded Human prostate tissue by Immunohistochemistry.
  • ab113941, at 1/50, staining ERCC1 in Paraffin-embedded Human bladder tissue by Immunohistochemistry.
  • ab113941, at 1/50, staining ERCC1 in Paraffin-embedded Human bladder carcinoma tissue by Immunohistochemistry.
  • ab113941, at 1/100, staining ERCC1 in COS7 cells transiently transfected by pCMV6-ENTRY ERCC1, by Immunofluorescence.
  • ab113941, at 1/100, staining ERCC1 in HeLa cells by Immunofluorescence.

Anti-ERCC1 antibody [4F9] (ab113941)参考文献

ab113941 has not yet been referenced specifically in any publications.

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