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Synthetic peptide conjugated to KLH derived from within residues 650 - 750 of Rat ERC2.
(Peptide available as ab27861.)
Our Abpromise guarantee covers the use of ab27250 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 111 kDa.|
|IHC-P||1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
Image courtesy of Human Protein Atlas
ab27250 staining ERC2 in Human cerebellum. The paraffin embedded human tissue was incubated with ab27250 (1/50 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6. Ab27250 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines.
Further results for this antibody can be found at www.proteinatlas.org
ab27250 has not yet been referenced specifically in any publications.