The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 3.75 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
功能Acts as an adapter for the receptor ERBB2, in epithelia. By binding the unphosphorylated 'Tyr-1248' of receptor ERBB2, it may contribute to stabilize this unphosphorylated state.
组织特异性Highly expressed in brain, heart, kidney, muscle and stomach, followed by liver, spleen and intestine.
序列相似性Belongs to the LAP (LRR and PDZ) protein family. Contains 17 LRR (leucine-rich) repeats. Contains 1 PDZ (DHR) domain.
细胞定位Cell junction > hemidesmosome. Found in hemidesmosomes, which are cell-substrate adhesion complexes in stratified epithelia. In transfected cells, either diffusely distributed over the cytoplasm or concentrated at the basolateral membrane.