RabMAb

Anti-ErbB 4 (phospho Y1284)抗体[EPR2273(2)] (ab109273)

概述

  • 产品名称Anti-ErbB 4 (phospho Y1284)抗体[EPR2273(2)]
    参阅全部 ErbB 4 一抗
  • 描述
    兔单克隆抗体[EPR2273(2)] to ErbB 4 (phospho Y1284)
  • 特异性This antibody detects Erb B4 phosphorylated at Tyrosine 1284 and may also detect Erb B2 phosphorylated at Tyrosine 1248.
  • 经测试应用适用于: WB, Flow Cyt, ICC/IFmore details
    不适用于: IHC-P or IP
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Phospho specific peptide corresponding to residues surrounding Tyrosine 1284 of Human ErbB 4.

  • 阳性对照
    • A431 cell lysate, treated with Epidermal Growth Factor (EGF); A431 cells; permeabilized EGF-treated A431 cells.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    A trial size is available to purchase for this antibody.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels.

    If you have any questions regarding this update, please contact our Scientific Support team.

性能

应用

Our Abpromise guarantee covers the use of ab109273 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Detects a band of approximately 180 kDa (predicted molecular weight: 147 kDa).
Flow Cyt 1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF 1/100 - 1/250.
  • 应用说明Is unsuitable for IHC-P or IP.
  • 靶标

    • 功能Specifically binds and is activated by neuregulins, NRG-2, NRG-3, heparin-binding EGF-like growth factor, betacellulin and NTAK. Interaction with these factors induces cell differentiation. Not activated by EGF, TGF-A, and amphiregulin. The C-terminal fragment (CTF) of isoform JMA-A CYT-2 (containing E4ICD2) can stimulate transcription in the presence of YAP1. ERBB4 intracellular domain is involved in the regulation of cell growth. Conflicting reports are likely due at least in part to the opposing effects of the isoform-specific and nuclear-translocated ERBB4 intracellular domains (E4ICD1 and E4ICD2). Overexpression studies in epithelium show growth inhibition using E4ICD1 and increased proliferation using E4ICD2. E4ICD2 has greater in vitro kinase activity than E4ICD1. The kinase activity is required for the nuclear translocation of E4ICD2.
    • 组织特异性Expressed at highest levels in brain, heart, kidney, in addition to skeletal muscle, parathyroid, cerebellum, pituitary, spleen, testis and breast. Lower levels in thymus, lung, salivary gland, and pancreas. Isoform JM-A CYT-1 and isoform JM-B CYT-1 are expressed in cerebellum, but only the isoform JM-B is expressed in the heart.
    • 序列相似性Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily.
      Contains 1 protein kinase domain.
    • 翻译后修饰Isoform JM-A CYT-1 and isoform JM-A CYT-2 but not isoform JM-B CYT-1 and isoform JM-B CYT-2 are processed by ADAM17. Proteolytic processing in response to ligand or 12-O-tetradecanoylphorbol-13-acetate stimulation results in the production of 120 kDa soluble receptor forms and intermediate membrane-anchored 80 kDa fragments (m80HER4), which are further processed by a presenilin-dependent gamma-secretase to release the respective cytoplasmic intracellular domain E4ICD (either E4ICD1/s80Cyt1 or E4ICD2/s80Cyt2). Membrane-anchored 80 kDa fragments of the processed isoform JM-A CYT-1 are more readily degraded by the proteasome than fragments of isoform JM-A CYT-2 suggesting a prevalence of E4ICD2 over E4ICD1.
      Ligand-binding increases phosphorylation on tyrosine residues. Isoform JM-A CYT-2 is constitutively phosphorylated on tyrosine residues in a ligand-independent manner. E4ICD2 but not E4ICD1 is phosphorylated on tyrosine residues.
      Ubiquitinated. The ERBB4 intracellular domain is ubiquitinated and targeted to proteosomal degradation during mitosis mediated by the APC/C complex. Isoform JM-A CYT-1 and isoform JM-B CYT-1 are ubiquitinated by WWP1. The ERBB4 intracellular domain (E4ICD1) is ubiquitinated, and this involves NEDD4.
    • 细胞定位Membrane and Nucleus. Following proteolytical processing E4ICD (E4ICD1 or E4ICD2 generated from the respective isoforms) is translocated to the nucleus. Significantly more E4ICD2 than E4ICD1 is found in the nucleus. E4ICD2 colocalizes with YAP1 in the nucleus.
    • Information by UniProt
    • 数据库链接
    • 别名
      • 4ICD antibody
      • ALS19 antibody
      • Avian erythroblastic leukemia viral oncogene homolog 4 antibody
      • Avian erythroblastic leukemia viral v erb b2 oncogene homolog 4 antibody
      • E4ICD antibody
      • EC 2.7.10.1 antibody
      • Erbb4 antibody
      • ERBB4 intracellular domain antibody
      • ERBB4_HUMAN antibody
      • HER 4 antibody
      • HER4 antibody
      • human epidermal growth factor receptor 4 antibody
      • Mer4 antibody
      • MGC138404 antibody
      • Oncogene ERBB4 antibody
      • p180erbB4 antibody
      • Proto-oncogene-like protein c-ErbB-4 antibody
      • Receptor protein tyrosine kinase erbB 4 precursor antibody
      • Receptor tyrosine protein kinase erbB 4 antibody
      • s80HER4 antibody
      • Tyrosine kinase type cell surface receptor HER4 antibody
      • Tyrosine kinase-type cell surface receptor HER4 antibody
      • v erb a avian erythroblastic leukemia viral oncogene homolog like 4 antibody
      • v erb a erythroblastic leukemia viral oncogene homolog 4 antibody
      • v-erb-a erythroblastic leukemia viral oncogene homolog 4 (avian) antibody
      • V-ERB-B2 avian erythroblastic leukemia viral oncogene homolog 4 antibody
      • Verba avian erythroblastic leukemia viral oncogene homolog like 4 antibody
      • Verba erythroblastic leukemia viral oncogene homolog 4 antibody
      • VERBB2 antibody
      see all

    Anti-ErbB 4 (phospho Y1284) antibody [EPR2273(2)] 图像

    • All lanes : Anti-ErbB 4 (phospho Y1284) antibody [EPR2273(2)] (ab109273) at 1/1000 dilution

      Lane 1 : A431 cell lysate, untreated
      Lane 2 : A431 cell lysate, treated with Epidermal Growth Factor (EGF)

      Lysates/proteins at 10 µg/ml per lane.


      Predicted band size : 147 kDa
    • ab109273 at 1/10 dilution staining ErbB 4 in HeLa cells by Flow cytometry.
      Green: Permeabilized A431 cells, untreated
      Red: Permeabilized A431 cells, treated with EGF.
      Blue: Permeabilized A431 cells, treated with EGF and preincubated with phospho-ErbB 4 (pY1284) peptide
      Orange: Permeabilized A431 cells, treated with EGF and preincubated with non-phospho-ErbB 4(pY1284) peptide.
    • Immunofluorescent staining of A431 cells using ab109273 at a dilution of 1/100.

    Anti-ErbB 4 (phospho Y1284) antibody [EPR2273(2)] (ab109273)参考文献

    This product has been referenced in:
    • Slanina H  et al. Role of epidermal growth factor receptor signaling in the interaction of Neisseria meningitidis with endothelial cells. Infect Immun 82:1243-55 (2014). Read more (PubMed: 24379285) »

    See 1 Publication for this product

    Product Wall

    Thank you for contacting us.

    The lab has sent me the following protocol and information:

    The ICC protocol used to test this antibody is attached. Cells are serum-starved for overnight, and then treated with 100 ng/ml EGF for 5 min a...

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    Thank you for your telephone call earlier today. According to Swissprot/Uniprot protein database site, the extracellular domain of the ErbB 4 is from amino acids 26 – 651. http://www.uniprot.org/uniprot/Q15303 We do have the following ...

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