重组
Anti-ErbB 4 (phospho Y1162)抗体[EP2270Y] (ab68478)
- 数据表
- 具体的参考文献 (1)
- 实验方案
概述
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产品名称Anti-ErbB 4 (phospho Y1162)抗体[EP2270Y]
参阅全部 ErbB 4 一抗 -
描述兔单克隆抗体[EP2270Y] to ErbB 4 (phospho Y1162)
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特异性This antibody detects ErbB 4 phosphorylated on tyrosine 1162.
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经测试应用适用于: WB, IP, IHC-Pmore details
不适用于: Flow Cyt or ICC/IF -
种属反应性与反应: Human
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免疫原
A phospho peptide corresponding to residues surrounding tyrosine 1162 of human ErbB 4.
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阳性对照
- A431 cell lysate, human breast carcinoma tissue.
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常规说明
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
A trial size is available to purchase for this antibody.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式Liquid
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存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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存储溶液pH: 7.40
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol, 0.05% BSA -
纯度Tissue culture supernatant
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克隆单克隆
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克隆编号EP2270Y
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同种型IgG
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研究领域
相关产品
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Positive Controls
应用
Our Abpromise guarantee covers the use of ab68478 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | ||
| IP | ||
| IHC-P |
应用说明
IHC-P: 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IP: 1/30.
WB: 1/25,000 - 1/50,000. Detects a band of approximately 200 kDa (predicted molecular weight: 147 kDa).
Is unsuitable for Flow Cyt or ICC/IF.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
IP: 1/30.
WB: 1/25,000 - 1/50,000. Detects a band of approximately 200 kDa (predicted molecular weight: 147 kDa).
Is unsuitable for Flow Cyt or ICC/IF.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
靶标
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功能Specifically binds and is activated by neuregulins, NRG-2, NRG-3, heparin-binding EGF-like growth factor, betacellulin and NTAK. Interaction with these factors induces cell differentiation. Not activated by EGF, TGF-A, and amphiregulin. The C-terminal fragment (CTF) of isoform JMA-A CYT-2 (containing E4ICD2) can stimulate transcription in the presence of YAP1. ERBB4 intracellular domain is involved in the regulation of cell growth. Conflicting reports are likely due at least in part to the opposing effects of the isoform-specific and nuclear-translocated ERBB4 intracellular domains (E4ICD1 and E4ICD2). Overexpression studies in epithelium show growth inhibition using E4ICD1 and increased proliferation using E4ICD2. E4ICD2 has greater in vitro kinase activity than E4ICD1. The kinase activity is required for the nuclear translocation of E4ICD2.
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组织特异性Expressed at highest levels in brain, heart, kidney, in addition to skeletal muscle, parathyroid, cerebellum, pituitary, spleen, testis and breast. Lower levels in thymus, lung, salivary gland, and pancreas. Isoform JM-A CYT-1 and isoform JM-B CYT-1 are expressed in cerebellum, but only the isoform JM-B is expressed in the heart.
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序列相似性Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily.
Contains 1 protein kinase domain. -
翻译后修饰Isoform JM-A CYT-1 and isoform JM-A CYT-2 but not isoform JM-B CYT-1 and isoform JM-B CYT-2 are processed by ADAM17. Proteolytic processing in response to ligand or 12-O-tetradecanoylphorbol-13-acetate stimulation results in the production of 120 kDa soluble receptor forms and intermediate membrane-anchored 80 kDa fragments (m80HER4), which are further processed by a presenilin-dependent gamma-secretase to release the respective cytoplasmic intracellular domain E4ICD (either E4ICD1/s80Cyt1 or E4ICD2/s80Cyt2). Membrane-anchored 80 kDa fragments of the processed isoform JM-A CYT-1 are more readily degraded by the proteasome than fragments of isoform JM-A CYT-2 suggesting a prevalence of E4ICD2 over E4ICD1.
Ligand-binding increases phosphorylation on tyrosine residues. Isoform JM-A CYT-2 is constitutively phosphorylated on tyrosine residues in a ligand-independent manner. E4ICD2 but not E4ICD1 is phosphorylated on tyrosine residues.
Ubiquitinated. The ERBB4 intracellular domain is ubiquitinated and targeted to proteosomal degradation during mitosis mediated by the APC/C complex. Isoform JM-A CYT-1 and isoform JM-B CYT-1 are ubiquitinated by WWP1. The ERBB4 intracellular domain (E4ICD1) is ubiquitinated, and this involves NEDD4. -
细胞定位Membrane and Nucleus. Following proteolytical processing E4ICD (E4ICD1 or E4ICD2 generated from the respective isoforms) is translocated to the nucleus. Significantly more E4ICD2 than E4ICD1 is found in the nucleus. E4ICD2 colocalizes with YAP1 in the nucleus.
- Information by UniProt
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数据库链接
- Entrez Gene: 2066 Human
- Omim: 600543 Human
- SwissProt: Q15303 Human
- Unigene: 390729 Human
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别名
- 4ICD antibody
- ALS19 antibody
- Avian erythroblastic leukemia viral oncogene homolog 4 antibody
see all
Anti-ErbB 4 (phospho Y1162) antibody [EP2270Y] 图像
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![Western blot - ErbB 4 (phospho Y1162) antibody [EP2270Y] (ab68478)](http://www.abcam.cn/ps/datasheet/Images/68/ab68478/ab68478wb.jpg)
Western blot - ErbB 4 (phospho Y1162) antibody [EP2270Y] (ab68478)All lanes : Anti-ErbB 4 (phospho Y1162) antibody [EP2270Y] (ab68478) at 1/500 dilution
Lane 1 : A431 cell lysate. Cells untreated.
Lane 2 : A431 cell lysate. Cells treated with EGF.
Lysates/proteins at 10 µg per lane.
Secondary
HRP conjugated Goat anti-Rabbit at 1/2000 dilution
Predicted band size : 147 kDa
Observed band size : 200 kDa (why is the actual band size different from the predicted?) -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - ErbB 4 (phospho Y1162) antibody [EP2270Y] (ab68478)](http://www.abcam.cn/ps/datasheet/Images/68/ab68478/ab68478ihc.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - ErbB 4 (phospho Y1162) antibody [EP2270Y] (ab68478)Human breast carcinoma stained with ab68478 at 1/100 dilution.
Anti-ErbB 4 (phospho Y1162) antibody [EP2270Y] (ab68478)参考文献
This product has been referenced in:
- Ranganathan S et al. Loss of EGFR-ASAP1 signaling in metastatic and unresectable hepatoblastoma. Sci Rep 6:38347 (2016). IHC-P ; Human . Read more (PubMed: 27910913) »
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"