Anti-ErbB 3 (phospho Y1289)抗体[EPR2325] (ab76469)


  • 产品名称Anti-ErbB 3 (phospho Y1289)抗体[EPR2325]
    参阅全部 ErbB 3 一抗
  • 描述
    兔单克隆抗体[EPR2325] to ErbB 3 (phospho Y1289)
  • 特异性ab76469 only detects ErbB 3 phosphorylated on Tyrosine 1289.
  • 经测试应用适用于: WB, ICC/IFmore details
    不适用于: Flow Cyt,IHC-P or IP
  • 种属反应性
    与反应: Human
  • 免疫原

    A phospho-specific peptide corresponding to residues surrounding Tyrosine 1289 of Human ErbB 3

  • 阳性对照
    • SKBR3 cell lysate treated with Neuregulin. MCF7 cells.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    A trial size is available to purchase for this antibody.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.



Our Abpromise guarantee covers the use of ab76469 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2500 - 1/5000. Detects a band of approximately 185 kDa (predicted molecular weight: 148 kDa).
ICC/IF 1/100.
  • 应用说明Is unsuitable for Flow Cyt,IHC-P or IP.
  • 靶标

    • 功能Binds and is activated by neuregulins and NTAK.
    • 组织特异性Epithelial tissues and brain.
    • 疾病相关Defects in ERBB3 are the cause of lethal congenital contracture syndrome type 2 (LCCS2) [MIM:607598]; also called Israeli Bedouin multiple contracture syndrome type A. LCCS2 is an autosomal recessive neurogenic form of a neonatally lethal arthrogryposis that is associated with atrophy of the anterior horn of the spinal cord. The LCCS2 syndrome is characterized by multiple joint contractures, anterior horn atrophy in the spinal cord, and a unique feature of a markedly distended urinary bladder. The phenotype suggests a spinal cord neuropathic etiology.
    • 序列相似性Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily.
      Contains 1 protein kinase domain.
    • 发展阶段Overexpressed in a subset of human mammary tumors.
    • 结构域The cytoplasmic part of the receptor may interact with the SH2 or SH3 domains of many signal-transducing proteins.
    • 翻译后修饰Ligand-binding increases phosphorylation on tyrosine residues and promotes its association with the p85 subunit of phosphatidylinositol 3-kinase.
    • 细胞定位Secreted and Cell membrane.
    • Information by UniProt
    • 数据库链接
    • 别名
      • c erbB 3 antibody
      • c erbB3 antibody
      • Erb b2 receptor tyrosine kinase 3 antibody
      • ErbB 3 antibody
      • ERBB3 antibody
      • ERBB3 protein antibody
      • erbB3 S antibody
      • ERBB3_HUMAN antibody
      • Glial growth factor receptor antibody
      • HER 3 antibody
      • HER3 antibody
      • Human epidermal growth factor receptor 3 antibody
      • LCCS2 antibody
      • MDA BF 1 antibody
      • MGC88033 antibody
      • p180 ErbB3 antibody
      • p45 sErbB3 antibody
      • p85 sErbB3 antibody
      • proto-oncogene-like protein c ErbB 3 antibody
      • proto-oncogene-like protein c ErbB3 antibody
      • Proto-oncogene-like protein c-ErbB-3 antibody
      • Receptor tyrosine protein kinase erbB 3 antibody
      • Receptor tyrosine protein kinase erbB3 antibody
      • Receptor tyrosine-protein kinase erbB-3 antibody
      • Tyrosine kinase type cell surface receptor HER3 antibody
      • Tyrosine kinase-type cell surface receptor HER3 antibody
      • v erb b2 avian erythroblastic leukemia viral oncogene homolog 3 antibody
      • v erb b2 erythroblastic leukemia viral oncogene homolog 3 (avian) antibody
      • v erb b2 erythroblastic leukemia viral oncogene homolog 3 antibody
      see all

    Anti-ErbB 3 (phospho Y1289) antibody [EPR2325] 图像

    • Immunofluorescent staining of MCF7 cells using ab76469 at 1/100 dilution.
    • All lanes : Anti-ErbB 3 (phospho Y1289) antibody [EPR2325] (ab76469) at 1/5000 dilution

      Lane 1 : SKBR3 cell lysate untreated
      Lane 2 : SKBR3 cell lysate treated with Neuregulin

      Lysates/proteins at 10 µg per lane.

      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 148 kDa
      Observed band size : 185 kDa (why is the actual band size different from the predicted?)

    Anti-ErbB 3 (phospho Y1289) antibody [EPR2325] (ab76469)参考文献

    This product has been referenced in:
    • Humtsoe JO  et al. ErbB3 upregulation by the HNSCC 3D microenvironment modulates cell survival and growth. Oncogene 35:1554-64 (2016). Read more (PubMed: 26073080) »
    • Sollome JJ  et al. HER2/HER3 regulates extracellular acidification and cell migration through MTK1 (MEKK4). Cell Signal 26:70-82 (2013). Human . Read more (PubMed: 24036211) »

    See all 3 Publications for this product

    Product Wall

    -Note yet tested on frozen sections but has been tested on paraffin embedded sections
    fixation methods: formalin
    Recommended starting dilution: 1:50
    Positive control: testis, brain
    Negative control: protein is widely ex...

    Read More

    Thank you for your enquiry. Unfortunately, we do not have a fluorescence conjugated version of this product. Also, a custom BSA-free production is not available.