Anti-ErbB 3 (phospho Y1289)抗体(ab101407)

概述

  • 产品名称Anti-ErbB 3 (phospho Y1289)抗体
    参阅全部 ErbB 3 一抗
  • 描述
    兔多克隆抗体to ErbB 3 (phospho Y1289)
  • 经测试应用适用于: ICC/IF, ELISA, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Cow, Macaque Monkey, Chinese Hamster, Orangutan
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1250 to the C-terminus of Human ErbB 3, phosphorylated at Y1289.

  • 阳性对照
    • This antibody gave a positive signal in both EGF treated A431 and MDA MB 361 whole cell lysates. This antibody gave a positive result when used in the following methanol fixed cell lines: MCF-7.

性能

应用

Our Abpromise guarantee covers the use of ab101407 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 µg/ml.
ELISA Use a concentration of 1 - 0.00025 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 200 kDa (predicted molecular weight: 148 kDa).

靶标

  • 功能Binds and is activated by neuregulins and NTAK.
  • 组织特异性Epithelial tissues and brain.
  • 疾病相关Defects in ERBB3 are the cause of lethal congenital contracture syndrome type 2 (LCCS2) [MIM:607598]; also called Israeli Bedouin multiple contracture syndrome type A. LCCS2 is an autosomal recessive neurogenic form of a neonatally lethal arthrogryposis that is associated with atrophy of the anterior horn of the spinal cord. The LCCS2 syndrome is characterized by multiple joint contractures, anterior horn atrophy in the spinal cord, and a unique feature of a markedly distended urinary bladder. The phenotype suggests a spinal cord neuropathic etiology.
  • 序列相似性Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily.
    Contains 1 protein kinase domain.
  • 发展阶段Overexpressed in a subset of human mammary tumors.
  • 结构域The cytoplasmic part of the receptor may interact with the SH2 or SH3 domains of many signal-transducing proteins.
  • 翻译后修饰Ligand-binding increases phosphorylation on tyrosine residues and promotes its association with the p85 subunit of phosphatidylinositol 3-kinase.
  • 细胞定位Secreted and Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • c erbB 3 antibody
    • c erbB3 antibody
    • Erb b2 receptor tyrosine kinase 3 antibody
    • ErbB 3 antibody
    • ERBB3 antibody
    • ERBB3 protein antibody
    • erbB3 S antibody
    • ERBB3_HUMAN antibody
    • Glial growth factor receptor antibody
    • HER 3 antibody
    • HER3 antibody
    • Human epidermal growth factor receptor 3 antibody
    • LCCS2 antibody
    • MDA BF 1 antibody
    • MGC88033 antibody
    • p180 ErbB3 antibody
    • p45 sErbB3 antibody
    • p85 sErbB3 antibody
    • proto-oncogene-like protein c ErbB 3 antibody
    • proto-oncogene-like protein c ErbB3 antibody
    • Proto-oncogene-like protein c-ErbB-3 antibody
    • Receptor tyrosine protein kinase erbB 3 antibody
    • Receptor tyrosine protein kinase erbB3 antibody
    • Receptor tyrosine-protein kinase erbB-3 antibody
    • Tyrosine kinase type cell surface receptor HER3 antibody
    • Tyrosine kinase-type cell surface receptor HER3 antibody
    • v erb b2 avian erythroblastic leukemia viral oncogene homolog 3 antibody
    • v erb b2 erythroblastic leukemia viral oncogene homolog 3 (avian) antibody
    • v erb b2 erythroblastic leukemia viral oncogene homolog 3 antibody
    see all

Anti-ErbB 3 (phospho Y1289) antibody 图像

  • ab101407 stained MCF-7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab101407 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • All lanes : Anti-ErbB 3 (phospho Y1289) antibody (ab101407) at 1 µg/ml

    Lane 1 : EGF-Stimulated A431 Whole Cell Lysate
    Lane 2 : MDA-MB-361 (Human breast adenocarcinoma cell line) Whole Cell Lysate Whole Cell Lysate
    Lane 3 : EGF-Stimulated A431 Whole Cell Lysate with Immunising peptide at 1 µg/ml
    Lane 4 : MDA-MB-361 (Human breast adenocarcinoma cell line) Whole Cell Lysate Whole Cell Lysate with Immunising peptide at 1 µg/ml
    Lane 5 : EGF-Stimulated A431 Whole Cell Lysate with Control peptide at 1 µg/ml
    Lane 6 : MDA-MB-361 (Human breast adenocarcinoma cell line) Whole Cell Lysate Whole Cell Lysate with Control peptide at 1 µg/ml

    Lysates/proteins at 15 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 148 kDa
    Observed band size : 200 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 33 kDa,42 kDa,60 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 8 minutes
    ErbB 3 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted. This blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab101407 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
  • ab101407 was tested using an Indirect ELISA approach. The wells were coated with peptide (1µg/ml at 100µl/well) overnight at 4°C, followed by a 5% BSA blocking step for 1 hour at room temperature. The primary Ab was then added at a dilution range of 1- 0.00025µg/ml (100µl/well) for 1hr at room temperature. A HRP-conjugated anti-rabbit IgG (heavy and light chain) was used as a secondary antibody at 1:20,000 dilution for 1hr at room temperature.

Anti-ErbB 3 (phospho Y1289) antibody (ab101407)参考文献

ab101407 has not yet been referenced specifically in any publications.

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