Anti-ErbB 3抗体[E186] (ab32121)


  • 产品名称Anti-ErbB 3抗体[E186]
    参阅全部 ErbB 3 一抗
  • 描述
    兔单克隆抗体[E186] to ErbB 3
  • 特异性ab32121 recognises ErbB3/HER3.
  • 经测试应用适用于: WBmore details
    不适用于: ICC/IF,IHC or IP
  • 种属反应性
    与反应: Rat, Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human ErbB 3 aa 1300 to the C-terminus (C terminal).
    Database link: P21860

  • 阳性对照
    • MCF-7 cell lysate.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.




Our Abpromise guarantee covers the use of ab32121 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 185 kDa (predicted molecular weight: 149 kDa).
  • 应用说明Is unsuitable for ICC/IF,IHC or IP.
  • 靶标

    • 功能Binds and is activated by neuregulins and NTAK.
    • 组织特异性Epithelial tissues and brain.
    • 疾病相关Defects in ERBB3 are the cause of lethal congenital contracture syndrome type 2 (LCCS2) [MIM:607598]; also called Israeli Bedouin multiple contracture syndrome type A. LCCS2 is an autosomal recessive neurogenic form of a neonatally lethal arthrogryposis that is associated with atrophy of the anterior horn of the spinal cord. The LCCS2 syndrome is characterized by multiple joint contractures, anterior horn atrophy in the spinal cord, and a unique feature of a markedly distended urinary bladder. The phenotype suggests a spinal cord neuropathic etiology.
    • 序列相似性Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily.
      Contains 1 protein kinase domain.
    • 发展阶段Overexpressed in a subset of human mammary tumors.
    • 结构域The cytoplasmic part of the receptor may interact with the SH2 or SH3 domains of many signal-transducing proteins.
    • 翻译后修饰Ligand-binding increases phosphorylation on tyrosine residues and promotes its association with the p85 subunit of phosphatidylinositol 3-kinase.
    • 细胞定位Secreted and Cell membrane.
    • Information by UniProt
    • 数据库链接
    • 别名
      • c erbB 3 antibody
      • c erbB3 antibody
      • Erb b2 receptor tyrosine kinase 3 antibody
      • ErbB 3 antibody
      • ERBB3 antibody
      • ERBB3 protein antibody
      • erbB3 S antibody
      • ERBB3_HUMAN antibody
      • Glial growth factor receptor antibody
      • HER 3 antibody
      • HER3 antibody
      • Human epidermal growth factor receptor 3 antibody
      • LCCS2 antibody
      • MDA BF 1 antibody
      • MGC88033 antibody
      • p180 ErbB3 antibody
      • p45 sErbB3 antibody
      • p85 sErbB3 antibody
      • proto-oncogene-like protein c ErbB 3 antibody
      • proto-oncogene-like protein c ErbB3 antibody
      • Proto-oncogene-like protein c-ErbB-3 antibody
      • Receptor tyrosine protein kinase erbB 3 antibody
      • Receptor tyrosine protein kinase erbB3 antibody
      • Receptor tyrosine-protein kinase erbB-3 antibody
      • Tyrosine kinase type cell surface receptor HER3 antibody
      • Tyrosine kinase-type cell surface receptor HER3 antibody
      • v erb b2 avian erythroblastic leukemia viral oncogene homolog 3 antibody
      • v erb b2 erythroblastic leukemia viral oncogene homolog 3 (avian) antibody
      • v erb b2 erythroblastic leukemia viral oncogene homolog 3 antibody
      see all

    Anti-ErbB 3 antibody [E186] 图像

    • Anti-ErbB 3 antibody [E186] (ab32121) at 1/1000 dilution + MDA-MB-435 (human ductal carcinoma) whole cell lysate at 10 µg

      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size : 149 kDa
    • Anti-ErbB 3 antibody [E186] (ab32121) at 1/500 dilution (unpurified) + MCF-7 cell lysate

      Predicted band size : 149 kDa
      Observed band size : 185 kDa (why is the actual band size different from the predicted?)

    Anti-ErbB 3 antibody [E186] (ab32121)参考文献

    This product has been referenced in:
    • Humtsoe JO  et al. ErbB3 upregulation by the HNSCC 3D microenvironment modulates cell survival and growth. Oncogene 35:1554-64 (2016). Read more (PubMed: 26073080) »
    • Ju JH  et al. HER2 stabilizes survivin while concomitantly down-regulating survivin gene transcription by suppressing Notch cleavage. Biochem J 451:123-34 (2013). Read more (PubMed: 23323858) »

    See all 5 Publications for this product

    Product Wall

    Application Western blot
    Loading amount 50 µg
    Gel Running Conditions Reduced Denaturing
    Sample Human Cell lysate - whole cell (iPSC-cardiomyocytes and lung adenocarcinoma cell l)
    Specification iPSC-cardiomyocytes and lung adenocarcinoma cell l
    Blocking step 1:1 Odyssey blocking buffer and 1X PBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100%

    Abcam user community

    Verified customer

    提交于 Aug 18 2014

    Thank you for your enquiry. I have been in touch with the source of this antibody and I have discussed the problems that you have been having with them. They consider the approach that you are proposing a good idea. They also suggested inducing the ...

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