Anti-ErbB3 / HER3抗体- C-terminal (ab190269)
Key features and details
- Rabbit polyclonal to ErbB3 / HER3 - C-terminal
- Suitable for: IP, WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-ErbB3 / HER3抗体- C-terminal
参阅全部 ErbB3 / HER3 一抗 -
描述
兔多克隆抗体to ErbB3 / HER3 - C-terminal -
宿主
Rabbit -
经测试应用
适用于: IP, WBmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Pig, Chimpanzee, Rhesus monkey, Chinese hamster, Orangutan -
免疫原
Synthetic peptide within Human ErbB3/ HER3 aa 1250 to the C-terminus (C terminal). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. NP_001973.2
Database link: P21860 -
阳性对照
- Hela, 293T, Jurkat, MCF-7 whole cell lysates
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7-8 -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab190269于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IP |
Use at 2-10 µg/mg of lysate.
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WB |
1/1000 - 1/5000. Predicted molecular weight: 148 kDa.
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说明 |
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IP
Use at 2-10 µg/mg of lysate. |
WB
1/1000 - 1/5000. Predicted molecular weight: 148 kDa. |
靶标
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功能
Binds and is activated by neuregulins and NTAK. -
组织特异性
Epithelial tissues and brain. -
疾病相关
Defects in ERBB3 are the cause of lethal congenital contracture syndrome type 2 (LCCS2) [MIM:607598]; also called Israeli Bedouin multiple contracture syndrome type A. LCCS2 is an autosomal recessive neurogenic form of a neonatally lethal arthrogryposis that is associated with atrophy of the anterior horn of the spinal cord. The LCCS2 syndrome is characterized by multiple joint contractures, anterior horn atrophy in the spinal cord, and a unique feature of a markedly distended urinary bladder. The phenotype suggests a spinal cord neuropathic etiology. -
序列相似性
Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily.
Contains 1 protein kinase domain. -
发展阶段
Overexpressed in a subset of human mammary tumors. -
结构域
The cytoplasmic part of the receptor may interact with the SH2 or SH3 domains of many signal-transducing proteins. -
翻译后修饰
Ligand-binding increases phosphorylation on tyrosine residues and promotes its association with the p85 subunit of phosphatidylinositol 3-kinase. -
细胞定位
Secreted and Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 451980 Chimpanzee
- Entrez Gene: 785655 Cow
- Entrez Gene: 100059216 Horse
- Entrez Gene: 2065 Human
- Entrez Gene: 13867 Mouse
- Entrez Gene: 100174431 Orangutan
- Entrez Gene: 100008807 Rabbit
- Entrez Gene: 29496 Rat
see all -
别名
- c erbB 3 antibody
- c erbB3 antibody
- Erb b2 receptor tyrosine kinase 3 antibody
see all
图片
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Immunoprecipitation of MCF-7 cell lysate (0.5-1 mg per IP reaction; 20% of IP loaded) labeling ErbB3 / HER3 with ab190269 at 1 µg/ml by Western blotting. Samples were immunoprecipitated using a control antibody (1), ab190269 at 6 μg per reaction (2), or control IgG (3)
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All lanes : Anti-ErbB3 / HER3 antibody - C-terminal (ab190269) at 0.4 µg/ml
Lane 1 : Hela whole cell lysate
Lane 2 : 293T whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lane 4 : MCF-7 whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 148 kDa
Exposure time: 30 seconds
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab190269 尚未被引用在任何文献中。