Anti-ErbB 2 (phospho Y1248)抗体(ab101229)

概述

  • 产品名称Anti-ErbB 2 (phospho Y1248)抗体
    参阅全部 ErbB 2 一抗
  • 描述
    兔多克隆抗体to ErbB 2 (phospho Y1248)
  • 经测试应用适用于: WB, ELISA, IHC-Pmore details
  • 种属反应性
    与反应: Rat, Human
  • 免疫原

    Synthetic peptide corresponding to Human ErbB 2 aa 1200 to the C-terminus (phospho Y1248) conjugated to Keyhole Limpet Haemocyanin (KLH).
    (Peptide available as ab139514)

  • 阳性对照
    • This antibody gave a positive signal in EGF-treated A431 whole cell lysate. IHC-P: FFPE human breast adenocarcinoma tissue sections.

性能

应用

Our Abpromise guarantee covers the use of ab101229 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 180 kDa (predicted molecular weight: 138 kDa).
ELISA Use at an assay dependent concentration.
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能Protein tyrosine kinase that is part of several cell surface receptor complexes, but that apparently needs a coreceptor for ligand binding. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Regulates outgrowth and stabilization of peripheral microtubules (MTs). Upon ERBB2 activation, the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane. This prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization.
    In the nucleus is involved in transcriptional regulation. Associates with the 5'-TCAAATTC-3' sequence in the PTGS2/COX-2 promoter and activates its transcription. Implicated in transcriptional activation of CDKN1A; the function involves STAT3 and SRC. Involved in the transcription of rRNA genes by RNA Pol I and enhances protein synthesis and cell growth.
  • 组织特异性Expressed in a variety of tumor tissues including primary breast tumors and tumors from small bowel, esophagus, kidney and mouth.
  • 疾病相关Hereditary diffuse gastric cancer
    Glioma
    Ovarian cancer
    Lung cancer
    Gastric cancer
    Chromosomal aberrations involving ERBB2 may be a cause gastric cancer. Deletions within 17q12 region producing fusion transcripts with CDK12, leading to CDK12-ERBB2 fusion leading to truncated CDK12 protein not in-frame with ERBB2.
  • 序列相似性Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily.
    Contains 1 protein kinase domain.
  • 翻译后修饰Autophosphorylated. Autophosphorylation occurs in trans, i.e. one subunit of the dimeric receptor phosphorylates tyrosine residues on the other subunit (Probable). Ligand-binding increases phosphorylation on tyrosine residues (PubMed:27134172). Signaling via SEMA4C promotes phosphorylation at Tyr-1248 (PubMed:17554007). Dephosphorylated by PTPN12 (PubMed:27134172).
  • 细胞定位Cytoplasm. Nucleus and Cell membrane. Cytoplasm, perinuclear region. Nucleus. Translocation to the nucleus requires endocytosis, probably endosomal sorting and is mediated by importin beta-1/KPNB1.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Verb b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog antibody
    • C erb B2/neu protein antibody
    • CD340 antibody
    • CD340 antigen antibody
    • Cerb B2/neu protein antibody
    • CerbB2 antibody
    • Erb b2 receptor tyrosine kinase 2 antibody
    • ERBB2 antibody
    • ERBB2_HUMAN antibody
    • HER 2 antibody
    • HER 2/NEU antibody
    • HER2 antibody
    • Herstatin antibody
    • Human epidermal growth factor receptor 2 antibody
    • Metastatic lymph node gene 19 protein antibody
    • MLN 19 antibody
    • MLN19 antibody
    • NEU antibody
    • NEU proto oncogene antibody
    • Neuro/glioblastoma derived oncogene homolog antibody
    • Neuroblastoma/glioblastoma derived oncogene homolog antibody
    • NGL antibody
    • p185erbB2 antibody
    • Proto-oncogene c-ErbB-2 antibody
    • Proto-oncogene Neu antibody
    • Receptor tyrosine-protein kinase erbB-2 antibody
    • TKR1 antibody
    • Tyrosine kinase type cell surface receptor HER2 antibody
    • Tyrosine kinase-type cell surface receptor HER2 antibody
    • V erb b2 avian erythroblastic leukemia viral oncogene homolog 2 (neuro/glioblastoma derived oncogene homolog) antibody
    • V erb b2 avian erythroblastic leukemia viral oncogene homolog 2 antibody
    • V erb b2 avian erythroblastic leukemia viral oncoprotein 2 antibody
    • V erb b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian) antibody
    • V erb b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog antibody
    • Verb b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian) antibody
    see all

Anti-ErbB 2 (phospho Y1248) antibody 图像

  • All lanes : Anti-ErbB 2 (phospho Y1248) antibody (ab101229) at 1 µg/ml

    Lane 1 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : EGF-Stimulated A431 Whole Cell Lysate
    Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate with Immunizing peptide at 1 µg/ml
    Lane 4 : EGF-Stimulated A431 Whole Cell Lysate with Immunizing peptide at 1 µg/ml
    Lane 5 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate with Non modified peptide at 1 µg/ml
    Lane 6 : EGF-Stimulated A431 Whole Cell Lysate with Non modified peptide at 1 µg/ml

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 138 kDa


    Exposure time : 3 minutes
    ErbB 2 (phospho Y1248)contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
  • IHC image of ab101229 staining in FFPE human breast adenocarcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab101229, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
  • ab101229 was tested using an Indirect ELISA approach. The wells were coated with peptide (1µg/ml at 100µl/well) overnight at 4°C, followed by a 5% BSA blocking step for 1 hour at room temperature. The primary Ab was then added at a dilution range of 1- 0.00025µg/ml (100µl/well) for 1hr at room temperature. A HRP-conjugated anti-rabbit IgG (heavy and light chain) was used as a secondary antibody at 1:20,000 dilution for 1hr at room temperature.

  • All lanes : Anti-ErbB 2 (phospho Y1248) antibody (ab101229) at 1/500 dilution

    Lane 1 : Schwann cell extract untreated
    Lane 2 : Schwann cell extract treated with 10 ng/ml GGF for 30 minutes.

    Lysates/proteins at 10 µg per lane.

    Secondary
    HRP conjugated goat anti-rabbit IgG at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 138 kDa
    Observed band size : 200 kDa (why is the actual band size different from the predicted?)


    Exposure time : 1 minute

    This image is courtesy of an anonymous Abreview

    Lanes on the left hand side show phosphorylation of ErbB2 at tyrosine 1248 (ab101299) in rat Schwann cells upon incubation with 10 ng/ml GGF for 30 minutes (lane 2).

    Treatment with GGF also induces phosphorylation of ErbB2 at tyrosine 877, which is observed on the right hand side blots.

    See Abreview

Anti-ErbB 2 (phospho Y1248) antibody (ab101229)参考文献

ab101229 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 10 µg
Gel Running Conditions Reduced Denaturing (10)
Sample Rat Cell lysate - whole cell (cultured Schwann cells)
Specification cultured Schwann cells
Treatment 10 ng/ml GCF for 30 mins (lane 2)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 25°C
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提交于 Sep 04 2013

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