RabMAb

Anti-ErbB 2 (phospho Y1221 + Y1222)抗体[EPR1047] (ab91633)

概述

  • 产品名称Anti-ErbB 2 (phospho Y1221 + Y1222)抗体[EPR1047]
    参阅全部 ErbB 2 一抗
  • 描述
    兔单克隆抗体[EPR1047] to ErbB 2 (phospho Y1221 + Y1222)
  • 特异性ab91633 detects ErbB 2 phosphorylated at tyrosine 1222.
  • 经测试应用适用于: WBmore details
    不适用于: Flow Cyt,ICC/IF,IHC-P or IP
  • 种属反应性
    与反应: Human
  • 免疫原

    Phosphospecific peptide corresponding to residues surrounding tyrosine 1222 of Human ErbB 2 (UniProt ID: P04626).

  • 阳性对照
    • SKBR3 cell lysate
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    A trial size is available to purchase for this antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

性能

应用

Our Abpromise guarantee covers the use of ab91633 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
  • 应用说明WB: 1/5000. Predicted molecular weight: 138 kDa.

    Is unsuitable for Flow Cyt, ICC/IF, IHC-P or IP.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Protein tyrosine kinase that is part of several cell surface receptor complexes, but that apparently needs a coreceptor for ligand binding. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Regulates outgrowth and stabilization of peripheral microtubules (MTs). Upon ERBB2 activation, the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane. This prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization.
      In the nucleus is involved in transcriptional regulation. Associates with the 5'-TCAAATTC-3' sequence in the PTGS2/COX-2 promoter and activates its transcription. Implicated in transcriptional activation of CDKN1A; the function involves STAT3 and SRC. Involved in the transcription of rRNA genes by RNA Pol I and enhances protein synthesis and cell growth.
    • 组织特异性Expressed in a variety of tumor tissues including primary breast tumors and tumors from small bowel, esophagus, kidney and mouth.
    • 疾病相关Hereditary diffuse gastric cancer
      Glioma
      Ovarian cancer
      Lung cancer
      Gastric cancer
      Chromosomal aberrations involving ERBB2 may be a cause gastric cancer. Deletions within 17q12 region producing fusion transcripts with CDK12, leading to CDK12-ERBB2 fusion leading to truncated CDK12 protein not in-frame with ERBB2.
    • 序列相似性Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily.
      Contains 1 protein kinase domain.
    • 翻译后修饰Autophosphorylated. Autophosphorylation occurs in trans, i.e. one subunit of the dimeric receptor phosphorylates tyrosine residues on the other subunit (Probable). Ligand-binding increases phosphorylation on tyrosine residues (PubMed:27134172). Signaling via SEMA4C promotes phosphorylation at Tyr-1248 (PubMed:17554007). Dephosphorylated by PTPN12 (PubMed:27134172).
    • 细胞定位Cytoplasm. Nucleus and Cell membrane. Cytoplasm, perinuclear region. Nucleus. Translocation to the nucleus requires endocytosis, probably endosomal sorting and is mediated by importin beta-1/KPNB1.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Verb b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog antibody
      • C erb B2/neu protein antibody
      • CD340 antibody
      • CD340 antigen antibody
      • Cerb B2/neu protein antibody
      • CerbB2 antibody
      • Erb b2 receptor tyrosine kinase 2 antibody
      • ERBB2 antibody
      • ERBB2_HUMAN antibody
      • HER 2 antibody
      • HER 2/NEU antibody
      • HER2 antibody
      • Herstatin antibody
      • Human epidermal growth factor receptor 2 antibody
      • Metastatic lymph node gene 19 protein antibody
      • MLN 19 antibody
      • MLN19 antibody
      • NEU antibody
      • NEU proto oncogene antibody
      • Neuro/glioblastoma derived oncogene homolog antibody
      • Neuroblastoma/glioblastoma derived oncogene homolog antibody
      • NGL antibody
      • p185erbB2 antibody
      • Proto-oncogene c-ErbB-2 antibody
      • Proto-oncogene Neu antibody
      • Receptor tyrosine-protein kinase erbB-2 antibody
      • TKR1 antibody
      • Tyrosine kinase type cell surface receptor HER2 antibody
      • Tyrosine kinase-type cell surface receptor HER2 antibody
      • V erb b2 avian erythroblastic leukemia viral oncogene homolog 2 (neuro/glioblastoma derived oncogene homolog) antibody
      • V erb b2 avian erythroblastic leukemia viral oncogene homolog 2 antibody
      • V erb b2 avian erythroblastic leukemia viral oncoprotein 2 antibody
      • V erb b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian) antibody
      • V erb b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog antibody
      • Verb b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian) antibody
      see all

    Anti-ErbB 2 (phospho Y1221 + Y1222) antibody [EPR1047] 图像

    • Primary antibody dilution: 1/1000

      Secondary antibody: goat anti-rabbit IgG, (H+L), peroxidase conjugated

      Secondary antibody dilution: 1/2500

      Blocking & dilution buffer: 5% NFDM/TBST

      Lane 1 sample: ErB 2 (pY1221/pY1222) phospho peptide

      Lane 2 sample: ErB 2 non-phospho peptide

      Exposure time: 3 minutes

    • All lanes : Anti-ErbB 2 (phospho Y1221 + Y1222) antibody [EPR1047] (ab91633) at 1/5000 dilution

      Lane 1 : SKBR3 cell lysate was untreated
      Lane 2 : SKBR3 cell lysate was treated with Lambda phosphatase.

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/1000 dilution

      Predicted band size : 138 kDa

    Anti-ErbB 2 (phospho Y1221 + Y1222) antibody [EPR1047] (ab91633)参考文献

    This product has been referenced in:
    • Humtsoe JO  et al. ErbB3 upregulation by the HNSCC 3D microenvironment modulates cell survival and growth. Oncogene 35:1554-64 (2016). Read more (PubMed: 26073080) »

    See 1 Publication for this product

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