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Our Abpromise guarantee covers the use of ab16899 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration.|
|IHC-Fr||Use a concentration of 5 µg/ml.|
|IP||Use a concentration of 1 µg/ml. Immunoprecipitates 185,000 dalton c-ErbB2/c-neu protein.|
|IHC-P||Use at an assay dependent concentration.|
|Flow Cyt||Use 0.1-1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.|
ab16899 at 1/100 staining human kidney cells by ICC/IF. The cells were fixed and permeabilized with acetone/methanol before being blocked with 50% serum for 5 minutes at 37°C. The primary antibody was then incubated for 30 minutes at 37°C. An Alexa Fluor® 488 goat anti-mouse antibody was used as the secondary.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"